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  4. Rabbit Complement fragment 3a,C3a ELISA Kit

Rabbit Complement fragment 3a,C3a ELISA Kit

  • 中文名稱：
    兔子補(bǔ)體片斷3a(C3a)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09283Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rabbit C3a ELISA Kit was designed for the quantitative measurement of Rabbit C3a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .

  • 縮寫：
    C3a
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %80-100
    1:2Average %96
    Range %85-104
    1:4Average %97
    Range %92-104
    1:8Average %90
    Range %82-98
  • 回收率：
    The recovery of rabbit C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9584-107
    EDTA plasma (n=4)9690-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    4002.591 2.620 2.606 2.460
    2001.889 1.874 1.882 1.736
    1001.328 1.320 1.324 1.178
    500.774 0.752 0.763 0.617
    250.434 0.441 0.438 0.292
    12.50.296 0.290 0.293 0.147
    6.250.254 0.244 0.249 0.103
    00.144 0.148 0.146 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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