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Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

  • 中文名稱:
    兔子Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E06858Rb
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Rabbit Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rabbit Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 別名:
    Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
  • 縮寫:
    Col Ⅰ
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 90  
    Range % 85-96  
    1:4 Average % 88  
    Range % 84-92  
    1:8 Average % 104  
    Range % 100-109  
  • 回收率:
    The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-96  
    EDTA plasma (n=4) 99 94-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.013 2.136 2.075 1.935  
    5 1.749 1.705 1.727 1.587  
    2.5 1.388 1.498 1.443 1.303  
    1.25 1.065 1.154 1.110 0.970  
    0.625 0.754 0.788 0.771 0.631  
    0.312 0.513 0.532 0.523 0.383  
    0.156 0.312 0.331 0.322 0.182  
    0 0.139 0.141 0.140    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價