Plant hormone abscisic acid,ABA ELISA Kit
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中文名稱:植物激素脫落酸(ABA)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09159Pl
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
植物激素脫落酸(ABA)是一種重要的植物激素,它可以影響植物的生長(zhǎng)、發(fā)育和對(duì)外界刺激的反應(yīng)。ABA具有保護(hù)作用,可以幫助植物應(yīng)對(duì)不利因素,如干旱、冷凍、逆境等。此外,ABA還能幫助控制植物生長(zhǎng)方向。
華美生物所提供的Plant hormone abscisic acid,ABA ELISA Kit屬于ELISA檢測(cè)試劑盒,采用競(jìng)爭(zhēng)法定量檢測(cè)植物植物組織樣本中的ABA,其靈敏度為0.04 μg/mL,檢測(cè)范圍為0.156 μg/mL-10 μg/mL。 -
縮寫:ABA
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種屬:Plant
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樣本類型:plant tissues
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檢測(cè)范圍:0.156 μg/ml-10 μg/ml.
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靈敏度:0.04 μg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<10% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<20% Three samples of known concentration were tested in twenty assays to assess. -
回收率:
The recovery of plant ABA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Xylem saps from plants (n=5) 95 89-98 Crude extracts (n=5) 95 90-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 10 0.124 0.126 0.125 5 0.213 0.228 0.221 2.5 0.357 0.361 0.359 1.25 0.498 0.506 0.502 0.625 0.686 0.701 0.694 0.312 1.244 1.268 1.256 0.156 1.510 1.534 1.522 0 2.241 2.297 2.269 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Disruption of Poly (ADP-ribosyl) ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymes NO Kalinina, N Spechenkova, I Ilina,International Journal of Molecular Sciences,2024
- Application of Synephrine to Grape Increases Anthocyanin via Production of Hydrogen Peroxide, Not Phytohormones M Suzuki,/,2024
- Yield improvement with antitranspirant application in droughted wheat associated with both reduced transpiration and reduced abscisic acid W Mphande,/,/
- Physiological and metabolomic responses of the ethylene insensitive squash mutant etr2b to drought J Iglesias-Moya,Plant science,2023
- Does the Grafting Affect Grapevine Rootstock Behavior Under Drought? G De Lorenzis,SSRN,2023
- Metabolic and film antitranspirants both reduce drought damage to wheat yield despite having contrasting effects on leaf ABA W Mphande,Journal of Agronomy and Crop Science,2023
- Ethylene-Insensitive Mutation Etr2b Confers Drought Tolerance in Squash by Promoting the Accumulation of Osmoprotectant and Antioxidant Metabolites M Jamilena,/,2023
- Physiological and Transcriptomic Evaluation of Drought Effect on Own-Rooted and Grafted Grapevine Rootstock (1103P and 101-14MGt) D Bianchi,Plants,2023
- The ethylene receptor mutation etr2b reveals crosstalk between ethylene and ABA in the control of Cucurbita pepo germination J Iglesias,Physiologia plantarum,2023
- Effects of antibiotics stress on root development, seedling growth, antioxidant status and abscisic acid level in wheat (Triticum aestivum L.) L Li,Ecotoxicology and environmental safety,2023
- Overexpression of a C3HC4-type RING E3 ligase gene, OsRFPHC-13, improves salinity resistance in rice, Oryza sativa, by altering the expression of Na+/K+ transporter genes JH Kim,Environmental and Experimental Botany,2023
- Metabolism of crown tissue is crucial for drought tolerance and recovery after stress cessation in Lolium/Festuca forage grasses D Perlikowski,Journal of Experimental Botany,2022
- Efficient Total Synthesis and Herbicidal Activity of 3-Acyltetramic Acids: Endogenous Abscisic Acid Synthesis Regulators X Liu,Journal of Agricultural and Food Chemistry,2022
- Pre- and Post-Harvest Conditions Affect Polyphenol Content in Strawberry (Fragaria × ananassa) R Koyama,Plants,2022
- Scavenging of nitric oxide up-regulates photosynthesis under drought in Festuca arundinacea and F. glaucescens but reduces their drought tolerance D Perlikowski,Scientific reports,2022
- Transcriptional reprogramming of the bud-mutation loquat YongLu emerging freezing resistant F Chen,Research Square,2020
- Yield improvement by antitranspirant application in droughted wheat is associated with reduced endogenous abscisic acid concentration W Mphande,Agricultural Water Management,2020
- Early events leading to water deficit responses in the liverwort Marchantia polymorpha D Godinez-Vidal,Environmental and Experimental Botany,2020
- Seasonal variations of epiphytic flora, abscisic acid production and physiological response in the brown alga Cystoseira foeniculacea (Linnaeus) Greville A KOZAK,Cah. Biol,2020
- Adjustment of photosynthetic activity to drought and fluctuating light in wheat M Grieco,Plant Cell Environ,2020
- Physiological and anatomical changes in tomato roots in response to low water stress LH Hernandez-Espinoza,Scientia Horticulturae,2020
- Efficient root metabolism improves drought resistance of Festuca arundinacea Perlikowski D, et al,Plant and Cell Physiology,2019
- An aquaporin gene MaPIP2-7 is involved in tolerance to drought, cold and salt stresses in transgenic banana (Musa acuminata L.) Xu Y, et al,Plant Physiology and Biochemistry,2019
- Structural and metabolic alterations in root systems under limited water conditions in forage grasses of Lolium-Festuca complex PerlikowskiDawid, et al,Plant science,2019
- The arginine decarboxylase gene ADC1, associated to the putrescine pathway, plays an important role in potato cold-acclimated freezing tolerance as revealed by transcriptome and metabolome analyses Kou S.et al,plant journal,2018
- Film antitranspirant application to enhance spring drought tolerance of oilseed rape (Brassica napus L.) Michele Faralli .et al,/,2018
- Comparative transcriptome analysis reveals an early gene expression profile that contributes to cold resistance in Hevea brasiliensis (the Para rubber tree) Cheng H.et al,Tree Physiol,2018
- Wilted cucumber plants infected by Fusarium oxysporum f. sp. cucumerinum do not suffer from water shortage Yuming Sun.et al,Annals of Botany,2017
- Canopy application of polymers over the reproductive phase enhances yield components and yield-related physiological traits of water stressed oilseed rape Michele Faralli.et al,Crop and Pasture Science,2016
- Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance. Zhou C.et al,Int J Mol Sci.,2016
- Overexpression of Rosa rugosa anthocyanidin reductase enhances tobacco tolerance to abiotic stress through increased ROS scavenging and modulation of ABA signaling. Luo P. et al,Plant Sci,2016
- Abscisic Acid and Gibberellin Control Seed Germination Through Negative Feedback Regulation By MOTHER OF FT AND TFL1 XI WANYAN ,Ph.D. Thesis,2010
- FTO-dependent function of N6-methyladenosine is involved in the hepatoprotective effects of betaine on adolescent mice Chen J. et al,J Physiol Biochem,2015
問(wèn)答及客戶評(píng)論
When the samples are ground, does it matter how quickly they are processed – I have hundreds of samples and I think I will have to grind at least 96/per kit before processing a set. Is the time taken after grinding before processing further limited, just in case the sample changes its chemical composition?
My samples are wheat leaves grown under drought conditions. Some of them are small collections and I am concerned they may not reach 0.5 g as stated in the protocol. If say the sample is less than 05.g, e.g. 0.4g, does that mean I will need to use 3.6 ml of the extraction buffer, since 0.5 g to 4.5 ml is 1: 9 in ratio terms.
It is recommended to grind the sample well and then process further on testing soon.
As you mentioned that you need to prepare large QTY sample, here advice you to store the first prepared sample at 2-8 ℃。 Pls kindly note this just can be stored for one day.
If you do not make the experiment at the same time, really advice you to prepare the sample partially according to your test request.
You can check your experiment schedule and control the time for this.
In theory, the sample volume can be adapted. Generally it is recommended to take 0.5g to prepare the sample. If it is too less, the sample will not be detected.
And dear, if your sample is really not abundant, here are 2 advice: first is that pls try to collect more sample, second is that change the sample based on the ratio terms 1:9.
The freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried – please state for how this needs to be. In addition, how is the grinding performed? What tools are used?
Yes, the freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried. You already keep them at -80 degrees Celsius, the samples are through the freeze-dried process indeed.
When do make the test, pls take 0.5g out and use the mortar usually used in lab to grind the freeze-dried samples.
And then check the detailed steps"Crude extracts " according to the manual to arrange the preliminary experiment.
靶點(diǎn)詳情
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最新研究進(jìn)展:植物激素脫落酸(Abscisic Acid,ABA)是一種重要的植物激素,具有調(diào)節(jié)植物生長(zhǎng)發(fā)育、應(yīng)對(duì)環(huán)境脅迫等多種生理作用。近年來(lái),對(duì)ABA的研究逐漸深入,以下是最新的研究進(jìn)展:ABA在植物抗旱適應(yīng)中的作用機(jī)制得到了深入研究。研究發(fā)現(xiàn),ABA能夠調(diào)節(jié)植物根系的生長(zhǎng)和分布,使根系更深入土壤中尋找水源。同時(shí),ABA還能夠促進(jìn)植物蒸騰作用的減緩,從而減少水分流失。ABA的代謝和信號(hào)轉(zhuǎn)導(dǎo)途徑研究取得了新進(jìn)展。研究人員已經(jīng)發(fā)現(xiàn)多種ABA受體,并且探索了它們與ABA的結(jié)合機(jī)制。同時(shí),ABA代謝途徑中的關(guān)鍵酶類的作用機(jī)制也得到了更深入的研究。ABA在植物與環(huán)境互動(dòng)中的作用機(jī)制也被深入探究。例如,研究發(fā)現(xiàn)ABA能夠調(diào)節(jié)植物對(duì)鹽堿脅迫的響應(yīng),同時(shí)還可以通過(guò)調(diào)節(jié)植物光合作用等途徑來(lái)增加植物對(duì)環(huán)境的適應(yīng)能力。ABA的應(yīng)用研究也取得了一定進(jìn)展。ABA已經(jīng)被廣泛用于植物生長(zhǎng)調(diào)節(jié)劑和抗旱劑等領(lǐng)域,例如在提高作物耐旱性、改善果實(shí)品質(zhì)等方面都有著廣泛的應(yīng)用前景。
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