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Pig free thyroxine (FT4) ELISA kit

  • 中文名稱:
    豬游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQF027512PI
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
        FT4(游離甲狀腺素):FT4是甲狀腺素中的游離形式,是人體代謝功能的重要調(diào)節(jié)因子之一。它是由甲狀腺腺體分泌的主要甲狀腺激素之一,與T3一樣,對(duì)細(xì)胞的能量代謝、生長(zhǎng)發(fā)育、心血管、神經(jīng)等系統(tǒng)的正常功能都有著重要的影響。FT4的含量水平是甲狀腺功能的重要指標(biāo),甲狀腺功能亢進(jìn)時(shí)FT4水平會(huì)升高,甲狀腺功能減退時(shí)FT4水平則會(huì)降低。
        華美生物所提供的Pig free thyroxine (FT4) ELISA kit屬于ELISA檢測(cè)試劑盒,采用競(jìng)爭(zhēng)法定量檢測(cè)豬 血清、血漿、細(xì)胞培養(yǎng)上清、組織勻漿樣本中的FT4,其靈敏度為1.08 pmol/L,檢測(cè)范圍為4 pmol/L-64 pmol/L。
     
  • 縮寫:
    FT4
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    4 pmol/L-64 pmol/L
  • 靈敏度:
    1.08 pmol/L
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 85-99    
    1:2 Average % 95    
    Range % 87-106    
    1:4 Average % 96    
    Range % 90-110    
    1:8 Average % 91    
    Range % 86-98    
  • 回收率:
    The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 93-103    
    EDTA plasma (n=4) 96 93-102    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pmol/L OD1 OD2 Average      
    4 2.771 2.736 2.754      
    8 1.459 1.442 1.451      
    16 0.827 0.865 0.846      
    32 0.575 0.565 0.570      
    64 0.318 0.307 0.313      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    FT4指的是游離甲狀腺素,是指在血液中不與蛋白質(zhì)結(jié)合的甲狀腺素。最新研究發(fā)現(xiàn),F(xiàn)T4水平與代謝綜合征、糖尿病、心血管疾病等疾病密切相關(guān)。一項(xiàng)針對(duì)14,107名美國(guó)成年人的研究表明,F(xiàn)T4水平與冠狀動(dòng)脈鈣化風(fēng)險(xiǎn)呈正相關(guān)關(guān)系。此外,一些研究表明,孕期FT4水平異常與胎兒神經(jīng)發(fā)育相關(guān)的基因表達(dá)水平有關(guān),提示FT4水平對(duì)胎兒神經(jīng)發(fā)育的影響。