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Pig Stromal cell derived factor 1a,SDF-1a ELISA Kit

  • 中文名稱:
    豬基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E12055p
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4500/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Pig SDF1a/CXCL12 ELISA Kit was designed for the quantitative measurement of Pig SDF1a/CXCL12 protein in serum, plasma, cell culture supernates, tissue homogenates, cell Lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 縮寫:
    SDF1a/CXCL12
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates, cell Lysates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %92
    Range %88-96
    1:4Average %86
    Range %82-90
    1:8Average %97
    Range %92-101
  • 回收率:
    The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-102
    EDTA plasma (n=4)8682-90
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.812 2.701 2.757 2.564
    102.397 2.361 2.379 2.186
    51.798 1.712 1.755 1.562
    2.51.011 0.979 0.995 0.802
    1.250.607 0.634 0.621 0.428
    0.6250.403 0.427 0.415 0.222
    0.3120.304 0.321 0.313 0.120
    00.188 0.198 0.193  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)