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Pig Immunoglobulin M,IgM ELISA Kit

  • 中文名稱:
    豬免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E06805p
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Pig IgM ELISA Kit was designed for the quantitative measurement of Pig IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.039 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

  • 縮寫:
    IgM
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.039 μg/mL-10 μg/mL
  • 靈敏度:
    0.039 μg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-103  
    1:2 Average % 87  
    Range % 81-92  
    1:4 Average % 95  
    Range % 90-99  
    1:8 Average % 102  
    Range % 96-108  
  • 回收率:
    The recovery of pig IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 104 98-108  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.204 0.206 0.205    
    2.5 0.463 0.454 0.459    
    0.62 0.915 0.936 0.926    
    0.16 1.429 1.488 1.459    
    0.04 2.056 2.134 2.095    
    0 2.515 2.652 2.584    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:其他 豬

稀釋比:沒有稀釋

產(chǎn)品評價: 標(biāo)曲準(zhǔn)確,樣本數(shù)值在標(biāo)曲范圍之內(nèi),符合預(yù)期結(jié)果。

By 謝老師