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Mouse thyroxine,T4 ELISA Kit

  • 中文名稱:
    小鼠甲狀腺素(T4)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E05083m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
        T4,全稱為甲狀腺素,是一種由甲狀腺分泌的激素,它在機(jī)體內(nèi)起到調(diào)節(jié)新陳代謝的作用,調(diào)控體溫、能量消耗和心臟、神經(jīng)系統(tǒng)的正常功能。T4進(jìn)入血液后會(huì)與血漿中的蛋白質(zhì)結(jié)合,主要是結(jié)合甲狀腺結(jié)合球蛋白,使其穩(wěn)定并運(yùn)輸至身體需要的地方。當(dāng)身體需要時(shí),T4會(huì)被轉(zhuǎn)化為T3,這是一種更加活躍的形式,可以進(jìn)入細(xì)胞并發(fā)揮作用。T4的水平可以通過(guò)血液測(cè)試來(lái)檢測(cè),這有助于診斷甲狀腺疾病和其他相關(guān)疾病。
        華美生物所提供的Mouse thyroxine,T4 ELISA Kit屬于ELISA檢測(cè)試劑盒,采用競(jìng)爭(zhēng)法定量檢測(cè)鼠血清、血漿、組織勻漿樣本中的T4,其靈敏度為20 ng/mL,檢測(cè)范圍為20 ng/mL-320 ng/mL。
     
  • 縮寫:
    T4
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    20 ng/mL-320 ng/mL
  • 靈敏度:
    20 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:

     

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 標(biāo)準(zhǔn)曲線:

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本試劑盒所含材料:
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody.
      • Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-conjugated T4 1 x 6 ml
      • HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

問(wèn)答及客戶評(píng)論

 常見(jiàn)問(wèn)題解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    近期研究發(fā)現(xiàn),T4蛋白在維持人類健康和免疫系統(tǒng)中發(fā)揮著重要的作用,特別是對(duì)于甲狀腺激素代謝的平衡具有重要作用。