This Mouse PDGFC ELISA Kit was designed for the quantitative measurement of Mouse PDGFC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
101
Range %
95-105
1:2
Average %
95
Range %
89-100
1:4
Average %
96
Range %
88-101
1:8
Average %
94
Range %
90-99
回收率:
The recovery of mouse PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
89
84-93
EDTA plasma (n=4)
98
90-103
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.
基因功能參考文獻(xiàn):
Results provide both in vitro and in vivo evidence that Mac-1 acting together with LRP1 facilitates PDGF-CC activation by thrombolytic tissue plasminogen activator in the neurovascular unit. The requirement of both Mac-1 and LRP1 for efficient activation of PDGF-CC by tPA provides a novel mechanism that helps limit PDGFRalpha signaling in the neurovascular unit. PMID: 28725968
High glucose-mediated induction of PDGF-C via ChREBP in mesangial cells contributes to the development of glomerular mesangial expansion in diabetes. PMID: 27033449
PDGF-CC neutralization or deficiency was not associated with preservation or accelerated loss of peritubular capillaries, suggesting no significant pro-angiogenic effects of PDGF-CC during renal fibrosis PMID: 26216283
heme oxygenase-1 (HMOX1) activity is critically required for the vascular protective/survival effect of PDGF-CC. PMID: 25267616
Studied survival and antiapoptotic effects of PDGF-C on focal retinal lesions in Ccl2(-/-)/Cx3cr1(-/-) on C57BL/6N [Crb1(rd8)] (DKO rd8) background mice, a model for progressive and focal retinal degeneration. PMID: 24709779
loss of FREM1 function promotes epidermal blistering in Fraser syndrome as a consequence of reduced PDGFC activity, in addition to its stabilising role in the basement membrane PMID: 24046351
Study indicates the role for PDGF-C as a critical regulator of impaired angiogenesis of diabetes. PMID: 23856609
High PDGF-C expression induces progressive fibrosis, chronic inflammation, neoangiogenesis and sinusoidal congestion resulting in hepatocellular carcinoma. PMID: 23929039
PDGF-C promotes tumor growth via a growth promoting effect on hepatic stellate cells that is dependent on the presence of functional PAK-2 PMID: 22362252
Data identified PDGF-CC as an important candidate target gene for antiangiogenic therapy, and PDGF-CC inhibition may be of therapeutic value in treating neovascular diseases. PMID: 20566880
PDGF-CC is critically required for neuronal survival in both brain and retina. Its neuroprotective effect of PDGF-CC is achieved by regulating GSK3beta phosphorylation and expression. PMID: 20231377
During development, PDGF-C expression was widespread and dynamic, and found in somites and their derivatives, in kidney, lung, brain PMID: 11744381
Dominant negative PDGF-C inhibits growth of Ewing family tumor cell lines. PMID: 12032822
Data show that platelet-derived growth factor-C is a potent mitogen for cardiac fibroblasts, and overexpression results in an expansion of the interstitium that induces a secondary sex-dependent hypertrophic response in the cardiomyocytes. PMID: 12875986
role for PDGF-C in bleomycin-induced pulmonary fibrosis. PMID: 12972405
During mouse fetal development, PDGF C was expressed in the mesonephric mesenchyme, prefusion skeletal muscle, cardiac myoblasts, and in visceral and vascular smooth muscle. PMID: 15061151
Pdgfc(-/-) mice die in the perinatal period owing to feeding and respiratory difficulties associated with a complete cleft of the secondary palate. PMID: 15361870
PDGF-C is activated during lung development and is a potent growth factor for mesenchymal cells in vivo. PMID: 16830225
These results suggest that PDGF-C signaling is a new mechanism of cleft palate induced by RA. PMID: 17066417
A method for the generation of conditional knockout alleles for Pdgfc is described. PMID: 17941048
PDGFC induced renal fibrosis involves PDGF-alpha receptor expressing cells and proinflammatory effects in renal interstitial fibrosis PMID: 18184860
some tumors may overcome inhibition of VEGF-mediated angiogenesis through upregulation of PDGF-C PMID: 19111878
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相關(guān)疾病:
Involved in the development of myocarditis and subsequent fibrosis in the experimental model of coxsackievirus B3-induced chronic myocarditis.
Mainly expressed in kidney, testis, liver, heart and brain (at protein level). Highly expressed in airway epithelium, interstitial cells and alveolar macrophages in the lung of mice overexpressing IL13. Expressed in the ovaries.