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  4. Mouse pancreatic alpha amylase (AMY2A)ELISA kit

Mouse pancreatic alpha amylase (AMY2A)ELISA kit

  • 中文名稱(chēng)：
    小鼠胰α淀粉酶(AMY2A)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001689MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse AMY2A ELISA Kit was designed for the quantitative measurement of Mouse AMY2A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.3 mU/mL.

  • 別名：
    Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
  • 縮寫(xiě)：
    AMY2A
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    9.38 mU/mL-600 mU/mL
  • 靈敏度：
    2.3 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-105
    1:2 Average % 92
    Range % 88-96
    1:4 Average % 86
    Range % 80-92
    1:8 Average % 89
    Range % 84-97
  • 回收率：
    The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-97
    EDTA plasma (n=4) 101 94-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    600 2.680 2.714 2.697 2.563
    300 2.164 2.235 2.200 2.066
    150 1.467 1.421 1.444 1.310
    75 0.759 0.811 0.785 0.651
    37.5 0.533 0.564 0.549 0.415
    18.75 0.317 0.326 0.322 0.188
    9.38 0.227 0.241 0.234 0.100
    0 0.128 0.139 0.134 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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