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  4. Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

  • 中文名稱：
    小鼠髓過氧化物酶特異性抗中性粒細胞胞質抗體酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08676m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：

    This Mouse MAC Ab ELISA Kit was designed for the quantitative measurement of Mouse MAC Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 縮寫：
    MAC Ab
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-102  
    1:2 Average % 95  
    Range % 83-101  
    1:4 Average % 96  
    Range % 90-106  
    1:8 Average % 94  
    Range % 82-101  
  • 回收率：
    The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA)spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 89-98  
    EDTA plasma (n=4) 94 91-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.404 2.418 2.411 2.297  
    20 1.956 1.904 1.930 1.816  
    10 1.352 1.377 1.365 1.251  
    5 0.902 0.934 0.918 0.804  
    2.5 0.526 0.534 0.530 0.416  
    1.25 0.311 0.326 0.319 0.205  
    0.625 0.265 0.271 0.268 0.154  
    0 0.112 0.115 0.114 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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