Mouse antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

This Mouse AVP ELISA Kit was designed for the quantitative measurement of Mouse AVP protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.31 pg/mL .
別名：

ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
縮寫：

AVP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

1.25 pg/mL-80 pg/mL
靈敏度：

0.31 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	87
	Range %	82-96
1:8	Average %	96
	Range %	90-104

回收率：

The recovery of mouse ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-94
EDTA plasma (n=4)	102	98-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.