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  4. Mouse Protein Wnt-16(WNT16) ELISA kit

Mouse Protein Wnt-16(WNT16) ELISA kit

  • 中文名稱(chēng)：
    小鼠Wnt-16蛋白(WNT16)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL026132MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse WNT16 ELISA Kit was designed for the quantitative measurement of Mouse WNT16 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 別名：
    Wnt16 ELISA Kit; Protein Wnt-16 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Stem Cells
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %103
    Range %101-105
    1:2Average %96
    Range %93-99
    1:4Average %89
    Range %86-92
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of mouse WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 106101-111
    EDTA plasma (n=4)9289-96
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.379 2.465 2.422 2.268
    20002.022 1.970 1.996 1.842
    10001.450 1.560 1.505 1.351
    5000.865 0.899 0.882 0.728
    2500.531 0.527 0.529 0.375
    1250.375 0.383 0.379 0.225
    62.50.288 0.278 0.283 0.129
    00.158 0.150 0.154
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 基因功能參考文獻(xiàn)：
    1. A large number of Mef2c targets overlapped with genes down-regulated by Wnt16 and Mef2c itself was transcriptionally repressed by Wnt16 suggesting that Mef2c plays a role in Wnt16-mediated transcriptional regulation. PMID: 29981832
    2. that WNT16 plays a critical role for acquisition of both cortical and trabecular bone mass and strength PMID: 28013361
    3. WNT16 antagonises excessive canonical WNT activation and protects cartilage in osteoarthritis. PMID: 27147711
    4. Wnt16 Is Associated with Age-Related Bone Loss and Estrogen Withdrawal in Murine Bone PMID: 26451596
    5. Describe TGFbeta-Wnt16-Notch signaling conduit in the chondrocyte-like transformation of VSMCs and identify endogenous TGFbeta activity in MGP-null VSMCs as a critical mediator of chondrogenesis. PMID: 25614285
    6. Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292
    7. These findings suggest that WNT16 acting via canonical WNT signaling regulates mechanical strain-induced periosteal bone formation and bone size. PMID: 25521583
    8. Osteoblast-derived WNT16 is a previously unreported key regulator of osteoclastogenesis and fracture susceptibility. PMID: 25306233
    9. Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/beta-catenin pathway. PMID: 24037946
    10. Study revealed new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. PMID: 19185060

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  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Wnt family
  • 數(shù)據(jù)庫(kù)鏈接：