Mouse Pro-Nerve growth factor (proNGF) ELISA kit
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中文名稱:小鼠神經(jīng)生長因子前體(proNGF)酶聯(lián)免疫試劑盒
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貨號:CSB-EQ027721MO
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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縮寫:proNGF
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:23.5 pg/mL-1500 pg/mL
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靈敏度:5.8 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse proNGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 91-99 1:2 Average % 102 Range % 97-106 1:4 Average % 85 Range % 81-89 1:8 Average % 94 Range % 90-98 -
回收率:
The recovery of mouse proNGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 97-106 EDTA plasma (n=4) 89 84-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1500 2.060 2.130 2.095 1.963 750 1.614 1.604 1.609 1.477 375 1.151 1.201 1.176 1.044 187.5 0.725 0.738 0.732 0.600 94 0.407 0.399 0.403 0.271 47 0.316 0.316 0.316 0.184 23.5 0.198 0.196 0.197 0.065 0 0.130 0.134 0.132 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- NGF-TrkA signaling dictates neural ingrowth and aberrant osteochondral differentiation after soft tissue trauma S Lee,Nature communications,2021
- RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival LilianKisiswa.et al,Cell reports,2018
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