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Mouse Osteocrin(OSTN) ELISA kit

  • 中文名稱：
    小鼠骨織素(OSTN)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL017269MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse OSTN ELISA Kit was designed for the quantitative measurement of Mouse OSTN protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 別名：
    Ostn ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
  • 縮寫：
    OSTN
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Stem Cells
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %92
    Range %87-97
    1:10Average %95
    Range %88-99
    1:20Average %93
    Range %87-100
    1:40Average %95
    Range %87-104
  • 回收率：
    The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9485-99
    EDTA plasma (n=4)9789-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.579 2.605 2.592 2.502
    5001.447 1.494 1.471 1.381
    2500.791 0.803 0.797 0.707
    1250.430 0.427 0.429 0.339
    62.50.219 0.225 0.222 0.132
    31.20.144 0.149 0.147 0.057
    15.60.107 0.112 0.110 0.020
    00.088 0.091 0.090
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

問(wèn)答及客戶評(píng)論

■ 常見(jiàn)問(wèn)題解答
Q:

Is an extraction step required from Plasma?

A:
Thanks for your inquiry! Extraction step is not required from Plasma.

靶點(diǎn)詳情

  • 功能：
    Hormone that acts as a ligand for natriuretic peptide receptor NPR3/NPR-C and promotes bone growth and physical endurance in muscle. Acts as a regulator of osteoblast differentiation and bone growth by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production. Required to enhance physical endurance: induced following physical exercise in muscle and promotes cGMP production, probably by interacting with NPR3/NPR-C. May act as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
  • 基因功能參考文獻(xiàn)：
    1. The mechanism underlying the effect of OSTN on skeletal growth relevant to C-type natriuretic peptide /Natriuretic peptide receptor B signaling. PMID: 28990933
    2. Data show that musclin (Ostn) production is stimulated by Ca2+-dependent proto-oncogene protein c-Akt (Akt) phosphorylation. PMID: 26668395
    3. findings suggest that palmitate increases musclin gene expression via the activation of the PERK signaling pathway in C2C12 myotubes PMID: 26449458
    4. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
    5. Musclin expression level is tightly regulated by nutritional changes and its physiological role could be linked to glucose metabolism PMID: 15044443
    6. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
    7. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Osteocrin family
  • 組織特異性：
    Expressed in skeletal muscle and to a much lesser extent in bone, brown adipose tissue, spleen and testis. Not expressed in neurons.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:239790

    UniGene: Mm.390880