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  4. Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit

Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit

  • 中文名稱：
    小鼠含EGF纖維蛋白細(xì)胞外基質(zhì)蛋白 1(EFEMP1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007450MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse EFEMP1 ELISA Kit was designed for the quantitative measurement of Mouse EFEMP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 別名：
    Efemp1 ELISA Kit; Fbln3EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Fibulin-3 ELISA Kit; FIBL-3 ELISA Kit
  • 縮寫：
    EFEMP1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 92-100
    1:2 Average % 88
    Range % 84-92
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 104
    Range % 100-108
  • 回收率：
    The recovery of mouse EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 85-94
    EDTA plasma (n=4) 97 93-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.243 2.168 2.206 2.033
    1000 1.553 1.605 1.579 1.406
    500 1.016 1.013 1.015 0.842
    250 0.597 0.574 0.586 0.413
    125 0.388 0.412 0.400 0.227
    62.5 0.324 0.337 0.331 0.158
    31.25 0.277 0.271 0.274 0.101
    0 0.177 0.169 0.173 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

問(wèn)答及客戶評(píng)論

■ 常見(jiàn)問(wèn)題解答
Q:

Regarding your ELISA kit CSB-EL007450MO, a customer is about to use it and they harvested their cells (for cell lysate samples) in RIPA buffer rather than PBS. Do you know if RIPA buffer would cause any negative effects with this kit, or is RIPA going to be expected to be OK?

A:
Thanks for your inquiry!
Generally speaking, RIPA will contain SDS, Triton X-100, NP-40 and other components. Customers now need to confirm the RIPA buffer formula. Pls note that it can not contain SDS (it will destroy the hydrogen bonds of the protein, which will lead to protein denaturation.);
For Triton X-100, NP-40, you need to control the low concentration range (generally not more than 1%, but it is not verified); For the entire RIPA buffer, sorry we have not tried this preparation method before, so we can only suggest you do a pretest if you want to try it.
Pls let me know if you have any further questions. Thank you.
Q:

Can you please advise on the immunogens used to raise the antibodies from this kit? Were they full-length or partial-length? And were the antibodies polyclonal or monoclonal?

A:
Thanks for your inquiry!CSB-EL007450MO
Capture antibody is rat monoclonal antibody and immunogen is NS0-derived recombinant protein,full-length.
Detection antibody is goat polyclonal antibody and immunogen is
NS0-derived recombinant protein,partial.

靶點(diǎn)詳情

  • 功能：
    Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
  • 基因功能參考文獻(xiàn)：
    1. TWEAK promotes migration and invasion in murine embryonic fibroblasts through a mechanism dependent on ERKs activation and Fibulin 3 down-regulation. PMID: 28383766
    2. Mutant fibulin-3 causes proteoglycan accumulation, reduction of MMP-2 and MMP-9, but increase of TIMP-3, and impairs diffusion across Bruch's membrane. PMID: 28622396
    3. Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560
    4. The Efemp1 knock-in mice reconstitute the most important histopathologic symptoms of both Malattia leventinese and age-related macular degeneration. PMID: 17664227
    5. fibulin-3 plays an important role in maintaining the integrity of fascia connective tissues and regulates aging. PMID: 17872905
    6. both fibulin-3 and -5 are important in maintaining pelvic organ support in mice. PMID: 19095964
    7. Data suggest a role for fibulin-3 in tissue maintenance and regeneration in the adult olfactory pathway. PMID: 19520159

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  • 亞細(xì)胞定位：
    Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 數(shù)據(jù)庫(kù)鏈接：