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  4. Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

  • 中文名稱：
    小鼠Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07925m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse Col Ⅲ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅲ protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 別名：
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 縮寫：
    Col Ⅲ
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 82-96  
    1:40 Average % 98  
    Range % 94-102  
    1:80 Average % 97  
    Range % 90-104  
    1:160 Average % 86  
    Range % 82-90  
  • 回收率：
    The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-99  
    EDTA plasma (n=4) 100 96-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    200 2.553 2.546 2.550 2.357  
    100 1.875 1.954 1.915 1.722  
    50 1.297 1.306 1.302 1.109  
    25 0.832 0.875 0.854 0.661  
    12.5 0.513 0.556 0.535 0.342  
    6.25 0.375 0.395 0.385 0.192  
    3.12 0.246 0.253 0.250 0.057  
    0 0.188 0.197 0.193 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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