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  4. Mouse Bone morphogenetic protein 2,BMP-2 ELISA Kit

Mouse Bone morphogenetic protein 2,BMP-2 ELISA Kit

  • 中文名稱：
    小鼠骨成型蛋白2(BMP-2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04509m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse BMP2 ELISA Kit was designed for the quantitative measurement of Mouse BMP2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 別名：
    Bmp2 ELISA Kit; Bmp-2 ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit; BMP-2A ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 88-101  
    1:2 Average % 101  
    Range % 95-105  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 99  
    Range % 91-103  
  • 回收率：
    The recovery of mouse BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 80-92  
    EDTA plasma (n=4) 101 92-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    100 2.643 2.453 2.548 2.423  
    50 2.024 1.976 2.000 1.875  
    25 1.349 1.312 1.331 1.206  
    12.5 0.742 0.724 0.733 0.608  
    6.25 0.449 0.467 0.458 0.333  
    3.12 0.307 0.300 0.304 0.179  
    1.56 0.242 0.237 0.240 0.115  
    0 0.126 0.124 0.125    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

問(wèn)答及客戶評(píng)論

■ 常見(jiàn)問(wèn)題解答
Q:

我打算使用貴公司的小鼠BMP-2 ELISA試劑盒，但其檢測(cè)范圍不適合我的研究。請(qǐng)問(wèn)我是否能使用人BMP-2 ELISA試劑盒來(lái)檢測(cè)小鼠BMP-2？我已比對(duì)了人和小鼠BMP-2的靶蛋白序列，發(fā)現(xiàn)它們100%同源。我正在研究小鼠細(xì)胞，想問(wèn)問(wèn)您的意見(jiàn)。

A:
不建議使用人BMP-2 ELISA Kit檢測(cè)小鼠細(xì)胞。這是考慮到不同試劑盒所使用的原料特性不同，不同樣本基質(zhì)也不同。如果您要測(cè)量小鼠來(lái)源的樣本，建議使用小鼠ELISA試劑盒。此外，如果樣本濃度非常低，則可能無(wú)法檢測(cè)到目的蛋白。關(guān)于小鼠細(xì)胞，不同來(lái)源和不同條件的樣本可能出現(xiàn)不同結(jié)果。如果您想使用小鼠試劑盒測(cè)試小鼠細(xì)胞，還需要進(jìn)行預(yù)實(shí)驗(yàn)并進(jìn)行評(píng)估。

靶點(diǎn)詳情

  • 功能：
    Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation. Stimulates also the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation.
  • 基因功能參考文獻(xiàn)：
    1. TNAP activation in vascular smooth muscle cells (VSMCs) appears sufficient to induce calcification. TNAP activation in VSMCs stimulates expression of chondrocyte markers. PMID: 27932058
    2. widespread myocardial Bmp2 and endocardial Notch signaling drive presumptive ventricular endocardium to differentiate into valve endocardium PMID: 29853617
    3. BMP-2 can enhance HUVEC proliferation, migration and angiogenesis through P38, ERK and Akt/m-TOR pathway. PMID: 27886213
    4. CTGF and BMP2 are induced following myocardial ischemia in mice and humans. PMID: 28460577
    5. Results indicate that Notch signaling induces cell cycle arrest and thereby initiates chondrocyte cell enlargement via bone morphogenetic protein 2 (BMP2) signaling. PMID: 27146698
    6. Collectively, our findings indicate that CBFA2T2 is required for BMP-2-induced osteogenic differentiation of MSCs through inhibition of EHMT1-mediated histone methylation at Runx2 promoter. PMID: 29378183
    7. The data suggest that SDF-1beta provides synergistic effects supporting BMP-2-induced, BMSC-mediated bone formation and appears suitable for optimization of bone augmentation in combination therapy protocols. PMID: 26227988
    8. study revealed that only BMP-6 was able to induce bone formation at the used dose and that the addition of IGF-1 contributed to an increase of the mineralization in the implants. Hence, the combination of BMP-6 with IGF-1 might be a better alternative than BMP-2 for orthopedic surgery or bone tissue engineering approaches. PMID: 28256809
    9. BMP2 may induce osteogenic differentiation. PMID: 28395271
    10. Increased bone mass in Crmp4(-/-) mice was associated with enhanced BMP2 signaling and BMP2-induced osteoblast differentiation in Crmp4(-/-) osteoblasts (OBs). PMID: 28019696
    11. Deletion of the "ultra-conserved sequence" within the 3'UTR of the Bmp2 was associated with elevated Bmp2 mRNA and BMP signaling levels, reduced fitness, and embryonic malformations. PMID: 28401685
    12. miR-106b inhibited osteoblastic differentiation and bone formation partly through directly targeting bone morphogenetic protein 2. PMID: 28108317
    13. cells stimulated with BMP-2 in the presence of FBS require the phosphorylation of Akt at Ser473 and the dephosphorylation of Akt at Thr308 to increase the osteoblast differentiation with alkaline phosphatase activity similar to that of BMP-9 plus FBS. PMID: 27477105
    14. This study showed that release of BMP-2 and SDF-1alpha from heparinized MCM scaffolds allows for the reduction of the applied BMP-2 concentration since SDF-1alpha seems to enhance the osteoinductive potential of BMP-2. PMID: 27060915
    15. mice implanted with sulfonated PRX/BMP-2 complexes exhibited rapid and significant bone regeneration compared to those implanted with free BMP-2 and heparin/BMP-2 complexes. PMID: 28130833
    16. these data demonstrate that in addition to BMP6, endothelial cell BMP2 has a non-redundant role in hepcidin regulation by iron. PMID: 28815688
    17. results indicate that Alx3 expression is enhanced by BMP-2 via the BMP receptors mediated-Smad signaling and that Alx3 is a positive regulator of osteoblast differentiation induced by BMP-2 PMID: 23825702
    18. KDM5A-mediated H3K4me3 modification participated in the etiology of osteoporosis and may provide new strategies to improve the clinical efficacy of BMP2 in osteoporotic conditions. PMID: 27512956
    19. BMP2 expression in the endocardial lineage is essential for the distal outgrowth, maturation and remodeling of atrioventricular endocardial cushions. PMID: 28790014
    20. the effect of titanium (Ti) with nanotopography (Nano) on the endogenous expression of BMP-2 and BMP-4 and the relevance of this process to the nanotopography-induced osteoblast differentiation. PMID: 26681207
    21. Dynamin-dependent endocytosis of Bone Morphogenetic Protein2 (BMP2) and its receptors is dispensable for the initiation of Smad signaling PMID: 27113717
    22. can increase [Ca(2+) ]i through extracellular calcium influx regulated by FAK and ALP and can deplete endoplasmic reticulum calcium through Src signaling simultaneously PMID: 26890302
    23. Our studies suggest operation of an auto/paracrine mechanism by which BMP-2 secreted by M1 macrophages maintains constitutive activation of a BMP-2 receptor/SMAD1/5 signaling axis. PMID: 28711495
    24. Genetic inactivation of hepatic angiocrine Bmp2 signaling in Stab2-Cre mice caused massive iron overload in the liver and increased serum iron levels and iron deposition in several organs similar to classic hereditary hemochromatosis; these changes were mediated by decreased hepatic expression of hepcidin, a key regulator of iron homeostasis. PMID: 27903529
    25. Actin cytoskeleton depolymerization inhibites BMP2 signaling via blocking of Smad by dephosphorylated CNN1 in osteoblast cells under simulated microgravity. PMID: 28457943
    26. Thus, our findings identify an unrecognized function of neogenin in mouse neocortical astrocyte differentiation, and suggest a signaling pathway, BMP2-neogenin-YAP-Smad1, underlying astrogliogenesis in developing mouse neocortex. PMID: 27225772
    27. These results suggest that BMP-2 directly induces Ifrd1 expression at the transcriptional level in osteoblasts via the Smad pathway, and Ifrd1 negatively regulates BMP-2-dependent osteoblastogenesis. PMID: 27856249
    28. By suppressing BMP2 signaling the adverse effects of excessive inflammation after myocardial infarction are limited. PMID: 27283840
    29. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells PMID: 27633082
    30. treatment of mBMSCs with the selective inhibitor of NF-kappaB pathway, BAY11-770682, showed to retrieve the inhibitory effect of CM-Adipo on BMP2-induced osteoblast differentiation in mBMSCs. PMID: 28173811
    31. BetA can enhance in vivo osteogenic potentials of BMP2, possibly via stimulating Smad 1/5/8 and p38 pathways, and combination of both agents can be considered as a therapeutic strategy for bone diseases. PMID: 27188281
    32. Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1-Smad5-Smad8 phosphorylation. PMID: 27030100
    33. Combining VEGF165 obviously enhanced the inducing effects of BMP2 on osteogenic differentiation capacity of C3H10T1/2 cells. PMID: 26757978
    34. Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation. PMID: 26635037
    35. Studied the effect of 7-dehydrocholesterol reductase (Dhcr7) and identified signal pathways involved in regulation of embryonic palatogenesis. Expression changes of Dhcr7, Sonic Hedgehog (Shh), and bone morphogenetic protein-2 (Bmp2) were measured by RT-PCR and WB after Dhcr7 gene silencing and the addition of exogenous cholesterol. PMID: 27066502
    36. A specific conserved AU-rich element within the Bmp2 ultra-conserved sequence was directly bound by the activating protein HuR. PMID: 26212702
    37. Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration. PMID: 26414873
    38. Following fracture, a CXCL12(+)-BMP2(+) perivascular cell population is recruited along the endosteum. PMID: 25967044
    39. Cyclic stretch enhanced the BMP-2induced osteoblastic differentiation through the inhibition of Hey1. PMID: 26647760
    40. Regulatory effects of fibroblast growth factor-8 and tumor necrosis factor-alpha on osteoblast marker expression induced by bone morphogenetic protein-2. PMID: 26409788
    41. assessed the role of BMP2 expression globally, by osteoblasts, and by vascular endothelial cells in endochondral healing, intramembranous healing and lamellar bone formation PMID: 26344756
    42. Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation PMID: 26214286
    43. these results suggest that the recently described nuclear variant of BMP2 is necessary for efficient secondary immune responses. PMID: 26491697
    44. only BMP7, not BMP2 or BMP4, is necessary for interdigital programmed cell death PMID: 26826495
    45. Gremlin blocks BMP2 signaling and function in pancreatic stellate cells in vitro PMID: 26141517
    46. These results demonstrated that the cotransfection of BMP-2 and VEGF into microencapsulated C2C12 cells is of potent utility for the potentiation of bone regeneration. PMID: 26451370
    47. alphavbeta3 integrin is required to mediate BMP-2-induced Smad signaling. PMID: 26953352
    48. Report phenotype of BMP2/4 double knockout osteoblast cell line. PMID: 26595646
    49. integrin alpha1beta1/BMPR IA may block BMP-2/BMPR IA complex information and interfere with the BMP-2 signalling pathway in cells PMID: 26475222
    50. Decreased expression of Osterix, as well as impaired TGFbeta and BMP2 signaling, contribute to the observed osteopenic bone phenotype of TIEG1 KO mice. PMID: 26801561

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 數(shù)據(jù)庫(kù)鏈接：