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  4. Human leukotriene E4,LT-E4 ELISA Kit

Human leukotriene E4,LT-E4 ELISA Kit

  • 中文名稱(chēng)：
    人白三烯E4(LTE4)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05176h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human LT-E4 ELISA Kit was designed for the quantitative measurement of Human LT-E4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 縮寫(xiě)：
    LT-E4
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Biochemicals
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LT-E4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 83-95  
    1:2 Average % 95  
    Range % 91-98  
    1:4 Average % 95  
    Range % 88-100  
    1:8 Average % 98  
    Range % 91-103  
  • 回收率：
    The recovery of human LT-E4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 81-93  
    EDTA plasma (n=4) 96 93-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.975 2.962 2.969 2.819  
    20 2.537 2.574 2.556 2.406  
    10 1.646 1.769 1.708 1.558  
    5 0.819 0.887 0.853 0.703  
    2.5 0.471 0.491 0.481 0.331  
    1.25 0.233 0.251 0.242 0.092  
    0.625 0.211 0.201 0.206 0.056  
    0 0.148 0.152 0.150 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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