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  4. Human hepatocyte growth factor activator(HGFA) ELISA kit

Human hepatocyte growth factor activator(HGFA) ELISA kit

  • 中文名稱：
    人肝細(xì)胞生長(zhǎng)因子激活物(HGFA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13160h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human HGFAC ELISA Kit was designed for the quantitative measurement of Human HGFAC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 別名：
    HGFAC ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
  • 縮寫：
    HGFAC
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %81-89
    1:2Average %95
    Range %91-99
    1:4Average %87
    Range %82-93
    1:8Average %105
    Range %101-109
  • 回收率：
    The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-91
    EDTA plasma (n=4)9491-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.117 2.148 2.133 2.025
    25001.696 1.682 1.689 1.581
    12501.215 1.268 1.242 1.134
    6250.834 0.816 0.825 0.717
    3120.502 0.536 0.519 0.411
    1560.265 0.258 0.262 0.154
    780.168 0.171 0.170 0.062
    00.106 0.109 0.108
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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靶點(diǎn)詳情

  • 功能：
    Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
  • 基因功能參考文獻(xiàn)：
    1. the HGF-activator has a role in multiple myeloma cells' catalysis of hepatocyte growth factor (HGF) activation PMID: 15172968
    2. HGF/MET signaling and aberrant HGF-activator expression is associated with diffuse large B-cell lymphoma PMID: 16189274
    3. KLK4 and KLK5 activate pro-HGFA. PMID: 18221492
    4. Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
    5. HAI-1 (serine peptidase inhibitor, Kunitz type 1) specifically traps active form of HGF activator on cellular surface and regulates HGF activation in pericellular microenvironment. PMID: 11013244

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  • 亞細(xì)胞定位：
    Secreted. Note=Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Liver.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4894

    OMIM: 604552

    KEGG: hsa:3083

    STRING: 9606.ENSP00000372224

    UniGene: Hs.104