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  4. Human apolipoprotein B100 (Apo-B100) ELISA Kit

Human apolipoprotein B100 (Apo-B100) ELISA Kit

  • 中文名稱：
    人載脂蛋白B100(Apo-B100)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08100h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        APOB（Apolipoprotein B）是一種脂蛋白，在人體中主要由肝臟合成，也可以由小腸上皮細(xì)胞合成。APOB參與膽固醇和甘油三酯的代謝和運輸，是載脂蛋白B100和B48的主要成分。APOB是膽固醇的主要運輸載體，通過結(jié)合在血漿中的膽固醇和甘油三酯形成不同類型的脂蛋白顆粒來運輸這些物質(zhì)到身體各部位。
        APOB與心血管疾病密切相關(guān)，過多的APOB會導(dǎo)致膽固醇沉積在血管壁上形成動脈粥樣硬化，進(jìn)而導(dǎo)致心血管疾病的發(fā)生。因此，APOB的水平可以作為心血管疾病的診斷和治療的重要生物標(biāo)記物和靶點。
        華美生物所提供的Human apolipoprotein B100 (Apo-B100) ELISA Kit屬于ELISA檢測試劑盒，采用競爭法定量檢測人血清、血漿、細(xì)胞培養(yǎng)上清、唾液、尿液樣本中的APOB，其靈敏度為19.5 ng/mL，檢測范圍為78 ng/mL-5000 ng/mL。
     
  • 別名：
    Apo B 100 ELISA Kit; Apo B ELISA Kit; Apo B-100 ELISA Kit; Apo B-48 ELISA Kit; Apo B100 ELISA Kit; Apo B48 ELISA Kit; ApoB 100 ELISA Kit; ApoB 48 ELISA Kit; APOB ELISA Kit; APOB_HUMAN ELISA Kit; Apolipoprotein B (including Ag(x) antigen) ELISA Kit; Apolipoprotein B 100 ELISA Kit; Apolipoprotein B 48 ELISA Kit; Apolipoprotein B ELISA Kit; Apolipoprotein B-48 ELISA Kit; Apolipoprotein B100 ELISA Kit; Apolipoprotein B48 ELISA Kit; FLDB ELISA Kit; LDLCQ4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, saliva, urine
  • 檢測范圍：
    78 ng/mL-5000 ng/mL
  • 靈敏度：
    19.5 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 96  
    Range % 92-102  
    1:400 Average % 95  
    Range % 91-99  
    1:800 Average % 104  
    Range % 102-108  
    1:1600 Average % 87  
    Range % 81-92  
  • 回收率：
    The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 92-102  
    EDTA plasma (n=4) 90 87-94  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    5000 0.074 0.073 0.074    
    2500 0.118 0.121 0.120    
    1250 0.182 0.172 0.177    
    625 0.272 0.284 0.278    
    312 0.451 0.442 0.447    
    156 0.770 0.754 0.762    
    78 1.122 1.054 1.088    
    0 2.371 2.270 2.321    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

Can you provide us with Human ApoB48 elisa kits?

A:
Thanks for your inquiry.
CSB-E08100h Human apolipoprotein B100 (Apo-B100) ELISA Kit is the proper kit for you. I will ask our distributor in your area to give you a quote.

靶點詳情

  • 最新研究進(jìn)展：
        最近的研究表明，APOB與心血管疾病風(fēng)險密切相關(guān)。一項薈萃分析研究發(fā)現(xiàn)，APOB水平與冠心病發(fā)病率呈正相關(guān)，提示APOB可能是冠心病的潛在生物標(biāo)記物。此外，其他研究還發(fā)現(xiàn)，APOB基因多態(tài)性與心血管疾病的風(fēng)險相關(guān)性增加，特別是在高膽固醇血癥和高血壓患者中。
        一些研究表明，APOB的表達(dá)水平可以通過改變飲食和藥物治療進(jìn)行調(diào)節(jié)，因此APOB可能成為心血管疾病的潛在治療靶點之一。
  • 功能：
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 基因功能參考文獻(xiàn)：
    1. Authors performed an analysis of public databases and literature for every variant published associated with FH, in the genes LDLR, APOB, and PCSK9. PMID: 29261184
    2. Targeted next generation DNA sequencing revealed several rare heterozygous missense variants in both MTTP and APOB genes known or predicted to be deleterious, in addition to a novel heterozygous missense variant in SAR1B, which encodes the gene causing chylomicron retention disease PMID: 29540175
    3. Single nucleotide polymorphisms rs693 and rs6725189 of the apoB gene are associated with calcific aortic stenosis in Chinese subjects, in Xinjiang, China. PMID: 29514644
    4. In conclusion, this study demonstrated that an APOB SNP, rs1042034, is closely associated with HCV infection through lipid metabolism alteration. PMID: 29382324
    5. APOB rs11279109 is associated with increased risk of coronary heart disease in Kuwaiti population. PMID: 29362515
    6. The simultaneous retention of fibrinogen and APOB-lipoproteins in FSD can be detected in routinely stained histological sections. The analysis of protein structures unraveled the pathomorphogenesis of this unexpected phenomenon. Fibrinogen gamma chain mutations provoke conformational changes in the region of the globular domain involved in the "end-to-end" interaction, thus impairing the D-dimer formation PMID: 29244742
    7. APOB polymorphism rs679899 is associated with type 2 diabetes and glutamyl transpeptidase levels, while the LIPC polymorphism rs6083 may influence plasma lipid levels in Chinese Han population. PMID: 29883758
    8. We present a case of homozygous familial defective apolipoprotein B-100 due to APOB R3500Q (rs5742904) treated with evolocumab ..Identification of a patient homozygous for familial defective apolipoprotein B-100(FDB) and successful treatment with PCSK9 inhibition PMID: 28988723
    9. Increased LDL levels were observed over time in patients with the -7673G>A polymorphism of the ApoB gene. PMID: 29846435
    10. Review/Meta-analysis: significant association of APOB rs1801701 GA, and APOB rs1042031 GA with increased risk of ischemic stroke. PMID: 28865324
    11. Serum apoB/A-I ratio appears to have value for predicting SAP in patients with AP. PMID: 28677336
    12. Apolipoprotein B is associated with carotid atherosclerosis progression PMID: 28826575
    13. Results show differences in the apoB100 secondary structure content among lipoproteins incubated at pH 7.4 or at pH 5.0 that could resemble those occurring in the early or late endosomes, respectively. PMID: 27824107
    14. Elevated serum apoB levels independently predict an increased risk for incident non-alcoholic fatty liver disease. PMID: 28106941
    15. This study shows that the ApoB-516C/T promoter gene polymorphism has no impact on the risk of hepatitis C virus infection. However, the C/T genotype may be a protective factor for females. PMID: 28370191
    16. association of TM6SF2 rs58542926 genotype with increased serum tyrosine levels and decreased apoB-100 particles in Finns PMID: 28539357
    17. that apos involved in TG metabolism such as apoC2, C3, E, and A4 (micromolar concentration), and apoB48 and apoA5 (single-digit nanomolar concentration) can be quantified from a single digestion mixture. PMID: 24694356
    18. These findings indicate that maternal apo B levels are significantly associated with apo B levels in their pre-school age children, adjusted for confounding variables. Furthermore, the mother-child correlations in apo B levels were independent of mother-child adiposity. Measurement of apo B levels in mothers may identify both high-risk children and mothers who may benefit from intervention. PMID: 28799493
    19. Individuals with apoB higher than predicted by non-HDL-C had significantly higher levels of PAI-1, which may contribute to the increased risk of future atherothrombotic events PMID: 28502501
    20. analysis of five likely pathogenic heterozygous rare variants that include four novel nonsense mutations in APOB (p.Gln845*, p.Gln2571*, p.Cys2933* and p.Ser3718*) and a rare variant in PCSK9 (Minor Allele Frequency <0.1%). PMID: 27179706
    21. In this sample of older adults with mild cognitive impairment, the presence of at least one copy of ApoE4 was associated with the development of both increased gait variability and cognitive decline during 1 year of follow-up PMID: 28482102
    22. The impact of smoking on the levels of apolipoprotein B (APOB) was evaluated by analyzing data from NHANES for the years 2007-2012 for US adolescents aged 12-19 years and adults aged greater than or eqult to 20 years. The study found that smoking did not influence the observed levels of APOB for either adolescents or adults. PMID: 28672200
    23. Identify LDLR, APOB and PCSK9 novel mutations causing familial hypercholesterolemia in the central south region of China. PMID: 28235710
    24. Rare variants of APOB or PCSK9 were identified in nine of the 22 study patients with extremely low LDL-C levels PMID: 29036232
    25. Eicosapentaenoic acid has direct antioxidant benefits in various apoB-containing subfractions. PMID: 26945158
    26. Association of plasma apoB with IR in obese subjects is dependent on gynoid WAT dysfunction PMID: 28391908
    27. Serum ApoB was not significantly different between term SGA newborns and control term newborns. PMID: 28304324
    28. APOB associated with Familial Hypercholesterolemia and Polygenic Hypercholesterolemia in patients with Acute Coronary Syndrome , age /=160 mg/dl. PMID: 28958330
    29. we identified common intronic SNPs (rs676210 and rs1042034) in the APOB gene and found five SNPs that were collectively associated with hyperlipidemia in the adult Chinese Yugur population. Importantly, we demonstrated that the G allele of rs676210 may confer an increased risk of hyperlipidemia. PMID: 28902930
    30. APOB missense variants, A224T and V925L, in a black South African woman with marked hypocholesterolemia PMID: 27206948
    31. male gender, ageing in women and menopause were associated with increased apoB concentrations. PMID: 27086565
    32. Data provide no evidence supporting an association between the APOB R3527Q variant and type 2 diabetes or glycemia. PMID: 28428224
    33. Levels of lipoprotein (a) [Lp(a)], a complex between an LDL-like lipid moiety containing one copy of apoB, and apo(a), a plasminogen-derived carbohydrate-rich hydrophilic protein, are primarily genetically rIncreasing evidence suggests that age, sex, and hormonal impact may have a modest modulatory influence on Lp(a) levels. Among clinical conditions, Lp(a) levels are reported to be affected by kidney and liver diseases. PMID: 26637279
    34. Data suggest that amphipathic beta-strands in 200 N-terminal residues of beta1 domain of APOB are required for secretion of lipid-rich or lipid-poor particles; residues 300-700 or 1050-1500 of beta1 domain appear to be required for secretion of lipid-rich particles; MTTP is required for secretion of intact APOB but not of truncated APOB. (APOB = apolipoprotein B; MTTP = microsomal triglyceride transfer protein) PMID: 28702990
    35. Basal apoB secretion by BeWo cells supports the concept of basal lipoprotein particle secretion by placental syncytiotrophoblast. PMID: 28487135
    36. ApoB EcoRI confers a moderate risk for coronary heart disease and the E(-) allele at this locus might be a susceptibility allele for the development of disease (Meta-Analysis) PMID: 27637205
    37. APOB single nucleotide polymorphism and it's association with LDL cholesterol level and risk of coronary heart disease in Pakistani population PMID: 27112212
    38. High apoB48 concentration is associated with Atherosclerotic Lesion Progression. PMID: 27487947
    39. Autoantibodies to oxidized lipoproteins and immune complexes with apoB-100 lipoproteins vary significantly by sex, age, and ethnicity. Higher baseline IgG MDA-LDL titers independently associate with new major adverse cardiovascular events. PMID: 28473443
    40. miR-548p regulates apoB secretion by targeting its mRNA degradation. PMID: 28336556
    41. Adolescent girls with obesity and polycystic ovary syndrome have elevated fasting and postprandial plasma triglycerides and ApoB-lipoprotein remnants. PMID: 27997268
    42. the associations of rs693 and rs562338 polymorphisms representing the Apolipoprotein B locus with endophenotypes (total cholesterol [TC] and high-density lipoprotein cholesterol) and phenotypes, were examined. PMID: 27683205
    43. ApoB100 is indispensable for HCV infection. ApoB is required for the generation of fully infectious HCV virions. PMID: 28018102
    44. a higher inflammatory status is associated with decreased triglyceride-rich-rich lipoprotein apoB-48 secretion among insulin resistant subjects PMID: 28183448
    45. Several protein markers were also deregulated in extracellular vesicless from bladder tumour patients, and our data suggest that the presence of ApoB in the 100,000 pellet is a clear marker for malignancy. PMID: 27751843
    46. 10 association studies for Han Chinese, with 1195 CHD patients and 1178 healthy subjects, we found that XbaI (rs693) polymorphisms in APOB were statistically significantly associated with Coronary Heart Disease in Han Chinese population PMID: 27172140
    47. We observed a significant interaction between Ins/Del ApoB genotype and dietary omega-3 PUFA intake with respect to BMI, WC, and obesity risk in both unadjusted (P = 0.007, P = 0.001, and P = 0.021, respectively) and adjusted (P = 0.007, P = 0.04, and P = 0.002, respectively) samples in NIDDM patients. PMID: 27210509
    48. Multiple novel LDLR and ApoB mutations have been identified in a-United Kingdom-based cohort with familial hypercholesterolemia. PMID: 26748104
    49. Data suggest that LDL-C/apoB ratio (ratio of plasma level of total cholesterol to plasma level of apolipoprotein B) is an important biomarker in overall evaluation of atherogenicity in obese, overweight, and normal-weight men; this study of qualitative changes in lipid/lipoprotein profile for early diagnosis of dyslipidemias leading to atherosclerosis was conducted in Russia. PMID: 27347637
    50. Mutations in APOB is associated with hypobetalipoproteinemia. PMID: 26916057

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  • 相關(guān)疾病：
    Hypobetalipoproteinemia, familial, 1 (FHBL1); Familial ligand-defective apolipoprotein B-100 (FDB)
  • 亞細(xì)胞定位：
    Cytoplasm. Secreted. Lipid droplet.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 603

    OMIM: 107730

    KEGG: hsa:338

    STRING: 9606.ENSP00000233242

    UniGene: Hs.120759