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  4. Human activated protein C,APC ELISA Kit

Human activated protein C,APC ELISA Kit

  • 中文名稱：
    人活化蛋白C(APC)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09909h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human APC ELISA Kit was designed for the quantitative measurement of Human APC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 別名：
    N/A
  • 縮寫：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 87-96  
    1:200 Average % 94  
    Range % 90-97  
    1:400 Average % 97  
    Range % 94-103  
    1:800 Average % 94  
    Range % 90-98  
  • 回收率：
    The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-94  
    EDTA plasma (n=4) 87 84-92  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    100 2.755 2.990 2.873 2.754  
    50 2.065 2.201 2.133 2.014  
    25 1.359 1.288 1.324 1.205  
    12.5 0.853 0.816 0.835 0.716  
    6.25 0.470 0.473 0.472 0.353  
    3.12 0.325 0.302 0.314 0.195  
    1.56 0.184 0.192 0.188 0.069  
    0 0.115 0.122 0.119    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價