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  4. Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

  • 中文名稱：
    人酒石酸鹽抵抗的酸性磷酸酶(TRAP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13415h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human TRAP ELISA Kit was designed for the quantitative measurement of Human TRAP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.47 ng/mL-30 ng/mL and the sensitivity is 0.12 ng/mL.

  • 別名：
    N/A
  • 縮寫：
    TRAP
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.47 ng/mL-30 ng/mL
  • 靈敏度：
    0.12 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TRAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-96  
    1:2 Average % 97  
    Range % 95-100  
    1:4 Average % 94  
    Range % 90-98  
    1:8 Average % 90  
    Range % 87-93  
  • 回收率：
    The recovery of human TRAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 89 84-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    30 2.498 2.476 2.487 2.304  
    15 2.074 2.098 2.086 1.903  
    7.5 1.519 1.459 1.489 1.306  
    3.75 0.901 0.945 0.923 0.740  
    1.87 0.572 0.587 0.580 0.397  
    0.94 0.425 0.438 0.432 0.249  
    0.47 0.378 0.362 0.370 0.187  
    0 0.182 0.184 0.183 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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