Human NGAL/MMP-9 complex ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

The human NGAL/MMP-9 complex ELISA kit is suitable for quantitatively determining human NGAL/MMP-9 complex in multiple samples, including serum, plasma, saliva, cell culture supernates, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human NGAL/MMP-9 complex levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

NGAL protects MMP-9 from proteolytic degradation and enhances its stability and enzymatic activities by binding and forming the NGAL/MMP-9 complex. The NGAL/MMP-9 complex is involved in the development and progression of cancer. NGAL/MMP-9 complex expression and activities have been detected in tissue and urine samples from patients with vascular anomalies and cancers. NGAL, MMP-9, and their complex NGAL/MMP-9 have been proposed as soluble biomarkers for numerous malignancies.
縮寫：

NGAL/MMP-9 complex
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.313 ng/mL-20 ng/mL
靈敏度：

0.029 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	91
Range %	87-98
1:20	Average %	95
Range %	90-102
1:40	Average %	93
Range %	87-99
1:80	Average %	94
Range %	86-105

回收率：

The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	94	87-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.