Human Long palate, lung and nasal epithelium carcinoma-associated protein 4(C20orf186) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

This Human C20orf186 ELISA Kit was designed for the quantitative measurement of Human C20orf186 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.
別名：

BPI fold-containing family B member 4 ELISA Kit; Bpifb4 ELISA Kit; C20orf186 ELISA Kit; dJ726C3.5 ELISA Kit; Gm1006 ELISA Kit; Ligand-binding protein RY2G5 ELISA Kit; Long palate; lung and nasal epithelium carcinoma-associated protein 4 ELISA Kit; LPLC4_HUMAN ELISA Kit; Lplunc4 ELISA Kit
縮寫：

C20orf186
Uniprot No.：

P59827
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C20orf186 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	98
	Range %	92-106
1:4	Average %	84
	Range %	81-89
1:8	Average %	91
	Range %	83-98

回收率：

The recovery of human C20orf186 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	93	88-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

■ 客戶評(píng)論

Tested application: ELISA
Test sample: Plasma (anticoagulant)
Species: Human
Review: The kit was easy to do, has showen significative difference between normal and treated groups, in range with expected values.
Tested application: ELISA
Test sample: Plasma
Species: Human
Review: We analyzed the amount of the protein in plasma of normal and frail human subjects. We have found non statistical significant trends. The test worked well, the standard curve was really good (0.99). We have to increase the statistical power by adding new sample. Internal duplicate gave similar results.

功能：

May have the capacity to recognize and bind specific classes of odorants. May act as a carrier molecule, transporting odorants across the mucus layer to access receptor sites. May serve as a primary defense mechanism by recognizing and removing potentially harmful odorants or pathogenic microorganisms from the mucosa or clearing excess odorant from mucus to enable new odorant stimuli to be received.
基因功能參考文獻(xiàn)：
1. LAV-BPIFB4 isoform modulates eNOS signalling through Ca2+/PKC-alpha-dependent mechanism. PMID: 28419216
2. In conclusion, BPIFB4 and CXCR4 expression classify long-lived individuals health status that correlates with maintained mononuclear cell migration. PMID: 28121621
3. Longevity-associated variant-BPIFB4 may represent a novel therapeutic tool to fight endothelial dysfunction and promote vascular reparative processes. PMID: 26034043
亞細(xì)胞定位：

Secreted. Cytoplasm.
蛋白家族：

BPI/LBP/Plunc superfamily, BPI/LBP family
組織特異性：

Expressed in nasal tissue.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 16179

OMIM: 615718

KEGG: hsa:149954

STRING: 9606.ENSP00000364632

UniGene: Hs.38961