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  4. Human Lipopolysaccharides,LPS ELISA Kit

Human Lipopolysaccharides,LPS ELISA Kit

  • 中文名稱：
    人脂多糖/內(nèi)毒素(LPS)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09945h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        LPS (Lipopolysaccharide) 是一種內(nèi)毒素，存在于許多革蘭陰性菌的細(xì)胞外膜中，它的存在對(duì)于細(xì)菌的生長和生存是必要的。LPS的主要作用是激活免疫系統(tǒng)，引起免疫細(xì)胞的炎癥反應(yīng)，同時(shí)也可以引發(fā)嚴(yán)重的炎癥反應(yīng)和休克。在體內(nèi)，LPS可以通過血液-腦屏障和血液-脊髓液屏障進(jìn)入中樞神經(jīng)系統(tǒng)，引起神經(jīng)炎癥反應(yīng)和神經(jīng)系統(tǒng)損傷。
        華美生物所提供的Human Lipopolysaccharides,LPS ELISA Kit屬于ELISA檢測試劑盒，采用雙抗夾心法定量檢測人血清、血漿、組織勻漿樣本中的LPS，其靈敏度為1.56 pg/mL，檢測范圍為6.25 pg/mL-400 pg/mL。
     
  • 縮寫：
    LPS
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-94  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 94  
    Range % 89-99  
  • 回收率：
    The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-97  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.809 2.960 2.885 2.720  
    200 2.227 2.366 2.297 2.132  
    100 1.515 1.556 1.536 1.371  
    50 0.789 0.755 0.772 0.607  
    25 0.432 0.470 0.451 0.286  
    12.5 0.303 0.325 0.314 0.149  
    6.25 0.235 0.232 0.234 0.069  
    0 0.164 0.165 0.165 　  
  • 本試劑盒所含材料：
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答
Q:

為什么不同的物種有不同的ELISA試劑盒。是因?yàn)長PS的變化嗎?我可以用人LPS ELISA檢測小鼠血漿中的LPS嗎?

A:
不建議跨物種檢測。因?yàn)椴煌锓N基質(zhì)是不同的。

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    LPS是一種脂多糖，存在于細(xì)菌細(xì)胞壁中，可引發(fā)嚴(yán)重的炎癥反應(yīng)。最近的研究表明，LPS可以激活多種免疫細(xì)胞，如單核細(xì)胞和樹突狀細(xì)胞，并誘導(dǎo)它們釋放多種炎癥介質(zhì)。此外，LPS也被認(rèn)為是引發(fā)多種疾病的重要因素之一，例如感染、腸道炎癥和自身免疫性疾病等。研究人員正在努力開發(fā)抑制LPS介導(dǎo)的免疫反應(yīng)的新型治療藥物。