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  4. Human Interferon alpha-inducible protein 27, mitochondrial(IFI27) ELISA kit

Human Interferon alpha-inducible protein 27, mitochondrial(IFI27) ELISA kit

  • 中文名稱：
    人干擾素α誘導蛋白-27，線粒體(IFI27)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011009HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human IFI27 ELISA Kit was designed for the quantitative measurement of Human IFI27 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL .

  • 別名：
    IFI27; Interferon alpha-inducible protein 27, mitochondrial; p27; Interferon alpha-induced 11.5 kDa protein; Interferon-stimulated gene 12a protein; ISG12(a; ISG12A
  • 縮寫：
    IFI27
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    28 pg/mL-1800 pg/mL
  • 靈敏度：
    7 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFI27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-99
    1:2Average %86
    Range %82-93
    1:4Average %94
    Range %89-98
    1:8Average %92
    Range %85-97
  • 回收率：
    The recovery of human IFI27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8784-90
    EDTA plasma (n=4)9995-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    18002.630 2.458 2.544 2.411
    9002.126 2.222 2.174 2.041
    4501.394 1.511 1.453 1.320
    2250.768 0.785 0.777 0.644
    1120.512 0.557 0.535 0.402
    560.342 0.321 0.332 0.199
    280.238 0.245 0.242 0.109
    00.134 0.131 0.133 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Probable adapter protein involved in different biological processes. Part of the signaling pathways that lead to apoptosis. Involved in type-I interferon-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9. Also functions in TNFSF10-induced apoptosis. May also have a function in the nucleus, where it may be involved in the interferon-induced negative regulation of the transcriptional activity of NR4A1, NR4A2 and NR4A3 through the enhancement of XPO1-mediated nuclear export of these nuclear receptors. May thereby play a role in the vascular response to injury. In the innate immune response, has an antiviral activity towards hepatitis C virus/HCV. May prevent the replication of the virus by recruiting both the hepatitis C virus non-structural protein 5A/NS5A and the ubiquitination machinery via SKP2, promoting the ubiquitin-mediated proteasomal degradation of NS5A.
  • 基因功能參考文獻：
    1. High IFI27 expression is associated with invasion in oral squamous cell carcinoma. PMID: 29580248
    2. NEAT1 and the coding gene IFI27 were highly co-expressed and negatively correlated with dengue severity. Monitoring NEAT1and IFI27 expression in PBMC may be useful in understanding dengue virus-induced disease progression. PMID: 29031635
    3. IFI27 was expressed in Influenza patients but not in Bacterial Respiratory Infection. IFI27 displayed 88% diagnostic accuracy (AUC) and 90% specificity in discriminating between Influenza and Bacterial Infections. PMID: 28619954
    4. ISG12a inhibited HCV replication and potentiated the anti-HCV activity of IFN-alpha possibly through induced production of type I IFNs and activation of Jak/STAT signaling pathway independent of autophagy and cell apoptosis. PMID: 27777077
    5. the ISG12 family of proteins has an important role for the apoptotic properties induced by type 1 interferon. PMID: 27673746
    6. IFI27 mRNA level is reduced in peripheral blood mononuclear cells of immunoglobulin A nephropathy and membranous nephropathy patients. PMID: 27100186
    7. IFI27 is involved in proliferation of skin keratinocytes both in vitro and in vivo. PMID: 25664647
    8. Data indicate that AKT control TRAIL resistance of cancer cells through downregulation of interferon stimulated gene 12a (ISG12a) by MicroRNA-942 (miR-942). PMID: 24970806
    9. We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. PMID: 25103640
    10. MiR-942 regulates hepatitis c virus-induced apoptosis of human hepatocytes by targeting ISG12a. PMID: 24727952
    11. These results suggest that high basal ISG12a may inhibit Newcastle disease virus replication and oncolysis, while low basal ISG12a may allow sufficient Newcastle disease virus replication for induction of ISG12a. PMID: 24999841
    12. Alterations in mechanisms associated with immune response and mitochondrial function that explain the up-regulation of IFI27 may provide an understanding of the pathways related to the intensification of fatigue during localized radiation therapy. PMID: 23959120
    13. After inhibiting the autophagy, the expression of interferon b1 and IFI27 were decreased. PMID: 23383510
    14. Patients with atypical teratoid/rhabdoid tumors show high p27 expression when angiogenesis and density of newly formed blood vessels were investigated. PMID: 22309998
    15. interferon alpha-inducible protein 27 (IFI27) transcription stimulation was weaker in leiomyoma than myometrial cells PMID: 22320196
    16. IFI27 may be a novel biomarker of disease activity and tumor burden in patients with chronic myeloproliferative neoplasms PMID: 21447007
    17. We identify IFI27, CCL2 and CXCL10 as sensitive biomarkers for the response of multiple sclerosis patients to IFN-beta. PMID: 19558503
    18. Assessment of p21, p27, Bax, and cyclin E expression in tumor tissues have been reported to be useful as prognostic factors in head and neck squamous cell carcinoma. PMID: 14719078
    19. Upregulated in whole blood of infants hospitalised with respiratory syncytial virus (RSV), subtype B, bronchiolitis. PMID: 17166282
    20. REsults describe the differential regulation of the interferon induced gene ISG12A by serum from healthy and preeclamptic pregnancies. PMID: 18329281
    21. data suggest that ISG12a contributes to IFN-dependent perturbation of normal mitochondrial function, thus adding ISG12a to a growing list of IFN-induced proteins that impact cellular apoptosis. PMID: 18330707
    22. five genes (TNFSF10/TRAIL, IL1RN, IFI27, GZMB, and CCR5) were upregulated and three genes (CLK1, TNFAIP3 and BTG1) were downregulated in at least three out of four subpopulations during acute GVHD. PMID: 18814951

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  • 亞細胞定位：
    Mitochondrion membrane; Multi-pass membrane protein. Nucleus inner membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    IFI6/IFI27 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5397

    OMIM: 600009

    KEGG: hsa:3429

    STRING: 9606.ENSP00000298902

    UniGene: Hs.532634