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  4. Human Fetuin A ELISA Kit

Human Fetuin A ELISA Kit

  • 中文名稱：
    人胎球蛋白A酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12882h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        胎球蛋白 A，也稱為 α-2-HS-糖蛋白 (AHSG)，是一種在肝臟中產(chǎn)生并在血液中循環(huán)的蛋白質(zhì)。它在血液凝固和鈣穩(wěn)態(tài)等幾個(gè)生物過程中起著重要作用，還已知對(duì)心血管疾病具有保護(hù)作用。
        華美生物所提供的Human Fetuin A ELISA Kit屬于ELISA檢測(cè)試劑盒，采用雙抗夾心法定量檢測(cè)人血清、血漿、細(xì)胞培養(yǎng)上清、組織勻漿、尿液樣本中的AHSG，其靈敏度為3.9 ng/mL，檢測(cè)范圍為15.6 ng/mL-1000 ng/mL。
     
  • 別名：
    59 kDa bone sialic acid-containing protein ELISA Kit; A2HS ELISA Kit; Aa2-066 ELISA Kit; AHS ELISA Kit; Ahsg alpha-2-HS-glycoprotein ELISA Kit; Ahsg ELISA Kit; Alpha 2 HS Glycoprotein ELISA Kit; Alpha 2 Z globulin ELISA Kit; Alpha-2-HS-glycoprotein ELISA Kit; Alpha-2-HS-glycoprotein chain B ELISA Kit; Alpha-2-Z-globulin ELISA Kit; Asialofetuin ELISA Kit; Ba alpha 2 glycoprotein ELISA Kit; Ba-alpha-2-glycoprotein ELISA Kit; BSP ELISA Kit; Countertrypin ELISA Kit; Fetua ELISA Kit; FETUA_HUMAN ELISA Kit; Fetuin ; mouse; homolog of ELISA Kit; Fetuin A ELISA Kit; Fetuin-A ELISA Kit; Glycoprotein PP63 ELISA Kit; HSGA ELISA Kit; pp63 ELISA Kit
  • 縮寫：
    AHSG
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, urine
  • 檢測(cè)范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 95  
    Range % 90-100  
    1:2000 Average % 94  
    Range % 91-99  
    1:4000 Average % 92  
    Range % 88-98  
    1:8000 Average % 93  
    Range % 84-102  
  • 回收率：
    The recovery of human fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-106  
    EDTA plasma (n=4) 98 91-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.754 2.797 2.776 2.564  
    500 2.312 2.354 2.333 2.121  
    250 1.563 1.554 1.559 1.347  
    125 0.735 0.753 0.744 0.532  
    62.5 0.538 0.526 0.532 0.320  
    31.2 0.322 0.335 0.329 0.117  
    15.6 0.268 0.284 0.276 0.064  
    0 0.211 0.213 0.212 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
        胎球蛋白 A (alpha-fetoprotein, AFP) 是一種在人類胚胎和胎兒發(fā)育期間表達(dá)的蛋白質(zhì)，在成年人中只有極低水平的表達(dá)。AFP是一種肝細(xì)胞癌的重要標(biāo)志物，也被廣泛用于孕期篩查和診斷胎兒畸形的指標(biāo)。
    以下是最近一些關(guān)于AFP的研究進(jìn)展：
        AFP在肝癌中的作用：AFP被認(rèn)為是肝癌干細(xì)胞的標(biāo)記物之一，因此在肝癌治療中具有重要作用。最近的研究表明，AFP通過調(diào)節(jié)Wnt/β-catenin信號(hào)通路來促進(jìn)肝癌細(xì)胞的增殖和轉(zhuǎn)移。
        AFP與自身免疫性疾病的關(guān)系：最近的研究表明，AFP可能與自身免疫性疾病的發(fā)生有關(guān)。研究人員發(fā)現(xiàn)，自身免疫性甲狀腺疾病患者血清中的AFP水平較高，這表明AFP可能與自身免疫性疾病的發(fā)生有關(guān)。
        AFP在胚胎干細(xì)胞和肝細(xì)胞再生中的作用：最近的研究表明，AFP在胚胎干細(xì)胞和肝細(xì)胞再生中起著重要作用。研究人員發(fā)現(xiàn)，AFP可以促進(jìn)胚胎干細(xì)胞向肝細(xì)胞的分化，并且在肝細(xì)胞再生過程中也發(fā)揮了重要作用。
        AFP在腫瘤免疫治療中的應(yīng)用：最近的研究表明，AFP可以作為腫瘤免疫治療的靶點(diǎn)之一。研究人員利用AFP的表達(dá)水平來選擇肝細(xì)胞癌患者進(jìn)行免疫治療，并且發(fā)現(xiàn)這種治療方法在一些患者中取得了良好的效果。
  • 功能：
    Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
  • 基因功能參考文獻(xiàn)：
    1. Serum Fetuin-A levels in septic patients decreased significantly in the first 24 hours, followed by an insignificant increase at 72 hours. PMID: 29968890
    2. Considering the role of fetuin-A in tissue calcification and inflammation, higher serum fetuin-A levels should be measured in patients with basal ganglia calcification PMID: 29288724
    3. This review is focused on work that has been done regarding fetuin-A potential role(s) in tumor progression and sequelae of diabetes. [review] PMID: 30060600
    4. In conclusion, Fetuin A, vitamin D, and intact PTH levels were all associated with sarcopenia in this geriatric population. Among them, intact PTH specifically indicates patients with sarcopenic left ventricular hypertrophy. PMID: 28112206
    5. fetuin a to adiponectin ratio is more strongly associated with metabolic syndrome and its components than fetuin-A or adiponectin alone PMID: 28779425
    6. High levels of fetuin-A and TGFbeta1 reported in patients with axial spondyloarthropathies. PMID: 26799059
    7. fetuin-A can be an alternative marker for insulin resistance and cardiovascular risk in prepubertal children PMID: 28154244
    8. rs4917 T allele carriers had higher triglycerides (TG). This relationship was exaggerated in individuals with overweight and obesity. Dietary intake was significantly associated with TG in only those with heterozygosity at rs4917, suggesting that these individuals may be more susceptible to dietary interventions. PMID: 28858873
    9. Although there is mechanistic evidence for an effect of fetuin-A on insulin sensitivity and secretion, data from this study does not support a strong, relevant relationship between circulating fetuin-A and risk of diabetes type 2 in the general European population studied. PMID: 29523632
    10. In overweight-to-obese older men, exercise/weight loss therapy appears to increase plasma fetuin-A levels, which is related to improvements in aerobic capacity. PMID: 28770230
    11. Higher fetuin-A levels are associated with incident type 2 diabetes in both males and females. PMID: 29439057
    12. Data suggest that plasma fetuin-A levels are down-regulated in type 2 diabetes with peripheral arterial disease, irrespective of traditional cardiovascular risk factors; fetuin-A may be involved in lower extremity arterial calcification in type 2 diabetes. PMID: 27899247
    13. Data suggest that, in children with type I diabetes, serum levels of osteoprotegerin are up-regulated, serum levels of RANKL are unchanged, and serum levels of fetuin-A are down-regulated. (RANKL = receptor activator of nuclear factor kappa B ligand) PMID: 27028343
    14. Elevated levels of pentraxin-3, fetuin-A, CRP, ESR might play a role in the pathogenesis of rheumatoid arthritis. Levels of fetuin-A, insulin HOMA-IR, pentraxin-3, CRP and ESR were not associated with clinical severity of the rheumatoid arthritis. PMID: 27152709
    15. the studies demonstrate the impact of fetuin-A as significant node of growth, motility, and invasion signaling in glioblastomas that can be targeted for therapy. PMID: 27882696
    16. Lower plasma fetuin-A levels were associated with an increased risk of all-cause and CVD mortality in patients with coronary artery disease independently of traditional CVD risk factors. PMID: 28912366
    17. thus creates an inflammatory environment in the pancreatic islets that can become a possible cause behind pancreatic beta-cell dysfunction in chronic hyperlipidemic condition PMID: 28797566
    18. In kidney disease, low circulating Fet-A concentrations are associated with progressive aortic stiffening and calcification. [review] PMID: 27190332
    19. The over-secretion of alpha-2-Heremans-Schmid glycoprotein by visceral adipose tissue, the increased secretion of the active phosphorylated form by subcutaneous adipose tissuein obese animals, and the adipose-derived alpha-2-Heremans-Schmid glycoprotein capacity to inhibit the insulin pathway suggest the participation of adipose-derived alpha-2-Heremans-Schmid glycoprotein in the deleterious effects of obesity. PMID: 27738888
    20. AHSG can be used to distinguish between inflammatory bowel disease and irritable bowel syndrome patients or healthy controls while at the same time "predicting" complicated disease behavior, need for therapy escalation and surgery. PMID: 28210079
    21. Lower baseline fetuin A levels and the presence of VC were independently linked to higher risk of incident fractures in prevalent dialysis patients PMID: 27398932
    22. present whole-exome sequencing results of a large consanguineous family segregating APMR syndrome with seven affected family members. Our study revealed a novel predicted pathogenic, homozygous missense mutation in the AHSG (OMIM 138680) gene PMID: 28054173
    23. fetuin A positively predicts circulating fetuin B in gestational diabetes mellitus PMID: 28183456
    24. Predictive role of the hepatokine fetuin A as a noninvasive biomarker of hepatic steatosis in preoperative settings. Compared to non-NAFLD subjects fetuin A was increased in NAFLD (p = .009) and in NASH (p = .029). PMID: 26980291
    25. The rs4918 minor variant is associated with lower TNFalpha and adiponectin, higher leptin levels in healthy persons, and more favorable anthropomorphic parameters of obesity. The G allele was more frequent among lean than obese patients (RR=1.067, 95%CI=1.053-2.651, p=0.015). An association between BMI and rs4918 polymorphism was observed among patients without diabetes but not in diabetics. PMID: 27487851
    26. fetuin-A does not have a role in the overall risk of cardiovascular disease PMID: 27038419
    27. Significant disturbance in serum and urinary fetuin-A levels, which was related to renal functions, had occurred during HDMTX and persisted for at least 4 months after the consolidation. Serum and urine fetuin-A could be sensitive markers for subtle renal dysfunction in ALL children. PMID: 26268515
    28. Serum fetuin-A level is not associated with disease progression and death and does not enhance risk prediction in non-dialysis chronic kidney disease stages 3-5. PMID: 27334353
    29. Increased serum visfatin, fetuin-A, and PTX3 levels, and the presence of positive correlation between visfatin, fetuin-A, and Psoriasis area and severity index, probably reflect the inflammatory state and insulin resistance seen in psoriasis. PMID: 25867925
    30. peritoneal dialysis patients with heart failure have significantly lower fetuin-A levels PMID: 24990392
    31. Study showed that serum levels of Fetuin-A are linked to serum calcium homeostasis and AHSG genotype but not with coronary artery calcification severity in subjects without coronary vasculopathy. PMID: 26825086
    32. Serum fetuin-A concentrations are independently correlated with VEGF and CRP concentrations in T2DM patients with DR, but not in NDR subjects PMID: 26851648
    33. From infancy to aging: Biological and behavioral modifiers of Fetuin-A. PMID: 26740309
    34. Fetuin-A exerts the stimulatory effects on inflammatory responses in HUVECs, macrophage foam cell formation, and proliferation and collagen production in HASMCs, leading to the development of atherosclerosis. PMID: 26828753
    35. Common variants in the AHSG gene are strongly associated with fetuin-A levels, but their concurrent association with CHD risk in current prospective studies is inconsistent PMID: 26343871
    36. results indicated that the cis-regulatory expressional difference of AHSG is expected to occur at the level of transcription or splicing of mRNA PMID: 26549924
    37. Data suggest that serum fetuin-A (FETUA/AHSG) concentrations are signi fi cantly positively associated with serum VEGFA (vascular endothelial growth factor A) levels in patients with newly diagnosed type 2 diabetes. PMID: 26156593
    38. Reanalysis of a total of 47 MDS patients revealed biomarker candidates, with alpha-2-HS-glycoprotein and leucine-rich alpha-2-glycoprotein as the most promising candidates. PMID: 26448929
    39. AHSG is up-regulated in hypopharyngeal squamous cell carcinoma may be employed as a potential biomarker for its early diagnosis PMID: 26464644
    40. The T nucleotide in rs4917 of AHSG is associated with more favorable lipid status among healthy persons (i.e., lower low-density lipoprotein cholesterol) and anthropologic parameters of obesity in cohort 2. PMID: 25695715
    41. In this large sample of community-dwelling older adults, a small positive association between fetuin-A and areal BMD appeared attributable to confounding variables and we found no evidence of an association between fetuin-A and risk of clinical fracture. PMID: 25656814
    42. The study findings showed that plasma sCD40L, fetuin-A, and PAPP-A levels are associated with carotid plaque formation and instability. PMID: 26214492
    43. There are increased carotid intima-media thickness values, decreased fetuin-A levels, but unchanged ABI values in patients with normotensive COPD. PMID: 23799555
    44. Fetuin-A is a novel risk marker for coronary artery disease in asymptomatic young adults. PMID: 25679296
    45. These findings establish urinary Fetuin-A as a sensitive biomarker of the progression of autosomal dominant polycystic kidney disease. PMID: 25888842
    46. Report increased secretion of Fetuin A, LBP and HMGB-1 from subcutaneous adipose tissue in metabolic syndrome. PMID: 25978344
    47. The present study demonstrates that fetuin-A 742 (C/T) and 766 (C/G) genotypes may be risk factors for myocardial infarction in patients older than 40 years of age. PMID: 25815703
    48. serum concentrations significantly associated with elevated fatty liver index, ALT, and AST, the early indicators of nonalcoholic fatty liver disease PMID: 26426614
    49. Circulating fetuin-A could be a useful serum biomarker for predicting liver and vascular fibrosis progression in fatty liver disease patients. PMID: 25627311
    50. Data indicate that the effect of testosterone on fetuin-A (alpha-2-HS-glycoprotein, AHSG) mRNA levels was abrogated by silencing of the androgen receptor in HepG2 cells. PMID: 25011927

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Fetuin family
  • 組織特異性：
    Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 349

    OMIM: 138680

    KEGG: hsa:197

    STRING: 9606.ENSP00000393887

    UniGene: Hs.324746