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Human Estradiol,E2 ELISA Kit

  • 中文名稱:
    人雌二醇(E2)酶聯免疫試劑盒
  • 貨號:
    CSB-E05108h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    雌二醇(Estradiol,E2)是一種雌激素,是雌性生殖系統發(fā)育和功能維持的重要激素。E2通過與細胞內的雌激素受體結合,調節(jié)多種生理過程,如生殖器官發(fā)育、月經周期、妊娠、乳腺發(fā)育等。除了生殖系統,E2還參與了神經、心血管、骨骼等系統的生理調節(jié)。E2在體內的水平受到多種因素的影響,如年齡、飲食、運動等。E2水平異常與多種疾病相關,如不孕、乳腺癌、骨質疏松等。E2的生理和病理作用受到廣泛關注,E2也被用于多種藥物的研發(fā)和治療。 華美生物所提供的Human Estradiol,E2 ELISA Kit屬于ELISA檢測試劑盒,采用競爭法定量檢測人血清、血漿樣本中的E2。
  • 別名:
    N/A
  • 縮寫:
    E2
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma.
  • 檢測范圍:
    50 pg/ml-1200 pg/ml.
  • 靈敏度:
    40 pg/ml.
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 本試劑盒所含材料:
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
    雌二醇(Estradiol,E2)是一種雌激素類固醇激素,廣泛存在于雌性生物體內。它通過與細胞表面的雌激素受體結合來調節(jié)細胞的生長、分化和代謝等生理過程,包括生殖發(fā)育、乳腺發(fā)育、內分泌平衡、骨骼代謝、心血管健康和認知功能等。最新的研究發(fā)現,E2還具有對中樞神經系統和免疫系統的影響,如對抗自身免疫疾病、改善老年認知障礙等具有潛在的治療作用。E2的異常水平與許多疾病的發(fā)生和發(fā)展有關,例如乳腺癌、卵巢癌、骨質疏松、心血管疾病、糖尿病和自身免疫疾病等。因此,E2的測量在臨床診斷和治療中具有重要的意義。