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  4. Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

  • 中文名稱：
    人EGF、嗜乳蛋白和7跨膜結(jié)構(gòu)域蛋白1(ELTD1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL007630HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human ELTD1 ELISA Kit was designed for the quantitative measurement of Human ELTD1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 別名：
    ADGRL4; ELTD1; ETL; UNQ202/PRO228; Adhesion G protein-coupled receptor L4; EGF, latrophilin and seven transmembrane domain-containing protein 1; EGF-TM7-latrophilin-related protein; ETL protein
  • 縮寫：
    ELTD1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ELTD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 94    
    Range % 89-98    
    1:2 Average % 97    
    Range % 91-103    
    1:4 Average % 92    
    Range % 87-98    
    1:8 Average % 88    
    Range % 85-92    
  • 回收率：
    The recovery of human ELTD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 101 98-104    
    EDTA plasma (n=4) 87 82-91    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1600 2.116 2.231 2.174 2.012    
    800 1.597 1.643 1.620 1.458    
    400 1.166 1.187 1.177 1.015    
    200 0.833 0.826 0.830 0.668    
    100 0.551 0.563 0.557 0.395    
    50 0.362 0.372 0.367 0.205    
    25 0.281 0.287 0.284 0.122    
    0 0.161 0.162 0.162 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Endothelial orphan receptor that acts as a key regulator of angiogenesis.
  • 基因功能參考文獻：
    1. High ELTD1 expression is associated with glioma. PMID: 27416955
    2. miR-139-5p suppresses glioblastoma multiforme cell proliferation by targeting ELTD1 and regulating cell cycle. PMID: 26449464
    3. Results provide insight into the regulation of tumor angiogenesis and highlight ELTD1 as key player in blood vessel formation. PMID: 23871637
    4. Two of these (D1S0818i-ELTD1 and D6S0035i-MAPK14) remain associated following application of Bonferroni's correction and multivariate analysis. PMID: 23474535
    5. Results of this study strongly suggests that associative analysis was able to accurately identify ELTD1 as a putative glioma-associated biomarker PMID: 23096411
    6. Genes such as ELTD1 on chromosome 1, in addition to genes on chromosomes 4 (eg, GABRA2) and 6 (eg, CNR1), may be associated with the genetic risk for cannabis use disorders. PMID: 18519829

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
  • 組織特異性：
    Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 20822

    OMIM: 616419

    KEGG: hsa:64123

    STRING: 9606.ENSP00000359778

    UniGene: Hs.132314