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  4. Human Cystatin-SN(CST1) ELISA kit

Human Cystatin-SN(CST1) ELISA kit

  • 中文名稱：
    人胱抑素SN(CST1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL006088HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human CST1 ELISA Kit was designed for the quantitative measurement of Human CST1 protein in serum, plasma, cell culture supernates,urine, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 別名：
    CST1 ELISA Kit; Cystain SAI ELISA Kit; Cystain-SA-I ELISA Kit; Cystatin 1 ELISA Kit; Cystatin-1 ELISA Kit; Cystatin-SN ELISA Kit; Cystatin1 ELISA Kit; Cysteine proteinase inhibitor, type 2 family ELISA Kit; CYTN ELISA Kit; CYTN_HUMAN ELISA Kit; Salivary cystatin SA1 ELISA Kit; Salivary cystatin-SA-1 ELISA Kit
  • 縮寫：
    CST1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates,urine, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CST1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 92-97  
    1:2 Average % 100  
    Range % 98-104  
    1:4 Average % 97  
    Range % 92-101  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human CST1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 93 90-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.406 2.389 2.398 2.278  
    5 1.841 1.776 1.809 1.689  
    2.5 1.246 1.223 1.235 1.115  
    1.25 0.695 0.685 0.690 0.570  
    0.625 0.464 0.444 0.454 0.334  
    0.312 0.252 0.249 0.251 0.131  
    0.156 0.187 0.185 0.186 0.066  
    0 0.122 0.118 0.120 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
  • 基因功能參考文獻(xiàn)：
    1. Results revealed that CST1 expression was upregulated in colon cancer (CC) compared with normal tissues and contributed to CC cell proliferation. PMID: 29845224
    2. High CST1 expression is negatively correlated with survival of breast cancer patients. CST1 promotes cell proliferation, clone formation, and metastasis in breast cancer cells. CST1 is a novel potential prognostic biomarker and therapeutic target for breast cancer. PMID: 28523467
    3. this results identified cystatin 1 as a biomarker in patients with seasonal allergic rhinitis PMID: 28633877
    4. These results indicate that CST1-mediated extracellular CatB activity enhances tumor development by preventing cellular senescence. PMID: 28383558
    5. interactions of human family 1 & 2 cystatins with cathepsin L1 PMID: 27764212
    6. high expression of Cystatin SN is a significant prognostic indicator of a higher rate of recurrence, metastatic risk, and poor survival in patients with surgically resected NSCLCs. PMID: 25648368
    7. In conclusion, our data suggest that CST1 might contribute to the proliferation of pancreatic cancer cells and could be a potential biomarker for the early detection of pancreatic cancer. PMID: 25577248
    8. CST1 may contribute to inactivation of protease allergens and help re-establish homeostasis of the nasal membranes. PMID: 23950865
    9. CST1 expression at both the messenger RNA and protein levels was barely detected in control cells, which included early passage proliferating, quiescent, or immortal human fibroblasts PMID: 21636832
    10. Murine monoclonal antibodies were made which can distinguish between CST1 and CST2. PMID: 15829315
    11. CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases. PMID: 19463800
    12. Identification of Cystatin SN as a novel tumor marker for colorectal cancer. PMID: 19513549

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 組織特異性：
    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2473

    OMIM: 123855

    KEGG: hsa:1469

    STRING: 9606.ENSP00000305731

    UniGene: Hs.123114