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Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

  • 中文名稱:
    人肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E05140h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Human CK-MB ELISA Kit was designed for the quantitative measurement of Human CK-MB protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.

  • 別名:
    N/A
  • 縮寫(xiě):
    CK-MB
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, urine, tissue homogenates
  • 檢測(cè)范圍:
    0.9 ng/mL-60 ng/mL
  • 靈敏度:
    0.22 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 87
    Range % 84-90
    1:2 Average % 104
    Range % 101-107
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 101
    Range % 98-104
  • 回收率:
    The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 85 81-89
    EDTA plasma (n=4) 109 104-113
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    60 2.016 2.106 2.061 1.907
    30 1.646 1.604 1.625 1.471
    15 1.215 1.253 1.234 1.080
    7.5 0.859 0.848 0.854 0.700
    3.75 0.586 0.552 0.569 0.415
    1.8 0.370 0.367 0.369 0.215
    0.9 0.284 0.278 0.281 0.127
    0 0.155 0.153 0.154  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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