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  4. Human Coll2-1 ELISA kit
  • 中文名稱(chēng)：
    人Coll2-1酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ027311HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human Coll2-1 ELISA Kit was designed for the quantitative measurement of Human Coll2-1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.

  • 縮寫(xiě)：
    Coll2-1
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %85
    Range %80-90
    1:200Average %98
    Range %90-105
    1:400Average %95
    Range %90-100
    1:800Average %92
    Range %87-97
  • 回收率：
    The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 83-95  
    EDTA plasma (n=4) 98 92-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    3000 2.513 2.487 2.500 2.417  
    1500 1.847 1.794 1.821 1.738  
    750 1.198 1.112 1.155 1.072  
    375 0.754 0.709 0.732 0.649  
    187.5 0.351 0.337 0.344 0.261  
    94 0.198 0.183 0.191 0.108  
    47 0.134 0.125 0.130 0.047  
    0 0.084 0.081 0.083 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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