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  4. Human Beta-Endorphin,β-EP ELISA Kit

Human Beta-Endorphin,β-EP ELISA Kit

  • 中文名稱：
    人β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06821h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Human β-EP ELISA Kit was designed for the quantitative measurement of Human β-EP protein in serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 別名：
    beta-Endorphin, beta-EP
  • 縮寫：
    β-EP
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %99
    Range %91-110
    1:4Average %97
    Range %92-105
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.537 2.568 2.553 2.443
    20002.010 1.999 2.005 1.895
    10001.600 1.532 1.566 1.456
    5001.032 1.122 1.077 0.967
    2500.567 0.600 0.584 0.474
    1250.391 0.410 0.401 0.291
    62.50.242 0.254 0.248 0.138
    00.109 0.111 0.110 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

我想咨詢一下人類β-內(nèi)啡肽ELISA試劑盒（CSB-E06821h）的問題。在規(guī)格說明書和使用手冊中，我不理解“靈敏度15.6 pg/mL”和“檢測范圍為62.5 pg/mL至4,000 pg/mL”的差異。在這個ELISA試劑盒中，15.6 pg/mL是最低可檢測水平嗎？如果是，為什么最低可檢測水平（15.6 pg/mL）低于范圍的最低水平（62.5 pg/mL）？

A:
L靈敏度15.6pg/m是該試劑盒的最低檢測水平，是通過重復測定20次零標準品OD值及其三倍標準差得出的。低于此參數(shù)（15.6pg/mL）則無法檢測。在15.6-62.5pg/mL區(qū)間范圍，樣品的測量值具有很大的變異性。62.5pg/mL 表示標準品S1的濃度點，而62.5-4000pg/mL是該試劑盒的檢測范圍。