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Human ADAMTS-like protein 2(ADAMTSL2) ELISA kit

  • 中文名稱:
    人ADAMTS樣蛋白2(ADAMTSL2)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL001318HU
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Human ADAMTSL2 ELISA Kit was designed for the quantitative measurement of Human ADAMTSL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 別名:
    ADAMTS like 2 ELISA Kit; ADAMTS like protein 2 ELISA Kit; ADAMTS-like protein 2 ELISA Kit; ADAMTSL 2 ELISA Kit; ADAMTSL-2 ELISA Kit; ADAMTSL2 ELISA Kit; ATL2_HUMAN ELISA Kit; FLJ45164 ELISA Kit; KIAA0605 ELISA Kit
  • 縮寫:
    ADAMTSL2
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTSL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-106
    1:2 Average % 88
    Range % 84-93
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 102
    Range % 98-106
  • 回收率:
    The recovery of human ADAMTSL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-96
    EDTA plasma (n=4) 86 81-92
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 1.854 1.988 1.921 1.812
    100 1.262 1.192 1.227 1.118
    50 0.780 0.733 0.757 0.648
    25 0.487 0.451 0.469 0.360
    12.5 0.312 0.334 0.323 0.214
    6.25 0.231 0.217 0.224 0.115
    3.12 0.169 0.160 0.165 0.056
    0 0.109 0.108 0.109  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

問答及客戶評論

 常見問題解答
Q:

我們正在尋找檢測ADAMTSL2的試劑盒,發(fā)現(xiàn)了CSB-EL001318HU。該試劑盒能否測量血漿或血清中的內(nèi)源性ADAMTSL2?該試劑盒使用的免疫原/校準劑是什么?是在某種表達系統(tǒng)中表達的全長重組蛋白么?

A:
本試劑盒的標準品為NS0衍生的重組蛋白。本試劑盒可檢測天然樣品(血清血漿等)中ADAMTSL2蛋白含量,但每種樣品的狀態(tài)不同,請檢查您自己的實際測量值。免疫原:NS0衍生的重組蛋白。

靶點詳情

  • 基因功能參考文獻:
    1. CpG-specific DNA methylation of ADAMTSL2 and BTN3A2 at rheumatoid arthritis diagnosis can serve as a marker of treatment response. PMID: 28447857
    2. Two compound heterozygous mutations were confirmed in the ADAMTSL2 gene of the patient with geleophysic dysplasia. PMID: 28917829
    3. A novel mutation in ADAMTSL2 (p. Gly421Ser) gene was identified in individuals with Ehlers-Danlos Syndrome. PMID: 26879370
    4. Two novel homozygous missense mutations in the ADAMTSL2 gene underlie geleophysic dysplasia in two consanguineous families from the United Arab Emirates. PMID: 24014090
    5. Molecular screening of ADAMTSL2 gene in 33 patients reveals the genetic heterogeneity of geleophysic dysplasia. PMID: 21415077
    6. These data suggest that ADAMTSL2 mutations may lead to a dysregulation of TGF-beta signaling and may be the underlying mechanism of geleophysic dysplasia. PMID: 18677313

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  • 相關疾?。?/div>
    Geleophysic dysplasia 1 (GPHYSD1)
  • 亞細胞定位:
    Secreted.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 14631

    OMIM: 231050

    KEGG: hsa:9719

    STRING: 9606.ENSP00000346478

    UniGene: Hs.522543