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Guinea pig insulin (INS) ELISA kit

  • 中文名稱：
    豚鼠胰島素(INS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011742GU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Guinea pig INS ELISA Kit was designed for the quantitative measurement of Guinea pig INS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12 μIU/mL-210 μIU/mL and the sensitivity is 7.5 μIU/mL.

  • 別名：
    INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    12 μIU/mL-210 μIU/mL
  • 靈敏度：
    7.5 μIU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-97  
    1:2 Average % 96  
    Range % 90-106  
    1:4 Average % 95  
    Range % 86-104  
    1:8 Average % 90  
    Range % 87-94  
  • 回收率：
    The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-101  
    EDTA plasma (n=4) 95 89-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    12 0.049 0.051 0.050    
    24 0.165 0.160 0.163    
    48 0.377 0.374 0.376    
    120 0.853 0.849 0.851    
    210 1.686 1.691 1.689    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：