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Goat adiponectin(ADP)ELISA Kit

  • 中文名稱(chēng)：
    山羊脂聯(lián)素(ADP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15981G
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Goat ADP ELISA Kit was designed for the quantitative measurement of Goat ADP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 縮寫(xiě)：
    ADP
  • Uniprot No.：
  • 種屬：
    Capra hircus (Goat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 95    
    Range % 89-100    
    1:2000 Average % 92    
    Range % 87-96    
    1:4000 Average % 99    
    Range % 93-104    
    1:8000 Average % 104    
    Range % 97-109    
  • 回收率：
    The recovery of goat ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 86-97    
    EDTA plasma (n=4) 94 87-97    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    20 0.192 0.190 0.191      
    10 0.406 0.383 0.395      
    5 0.692 0.656 0.674      
    2.5 1.010 0.974 0.992      
    1.25 1.426 1.389 1.408      
    0.625 1.930 1.872 1.901      
    0.312 2.232 2.315 2.274      
    0 2.463 2.400 2.432      
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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