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Goat Interleukin 10,IL-10 ELISA Kit

  • 中文名稱:
    山羊白介素10(IL-10)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E09809g
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Goat IL10 ELISA Kit was designed for the quantitative measurement of Goat IL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 縮寫:
    IL-10
  • Uniprot No.:
  • 種屬:
    Capra hircus (Goat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %82
    Range %80-85
    1:2Average %102
    Range %97-107
    1:4Average %89
    Range %84-93
    1:8Average %107
    Range %103-111
  • 回收率:
    The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8580-90
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.725 2.768 2.747 2.615
    10002.059 2.000 2.030 1.898
    5001.321 1.368 1.345 1.213
    2500.741 0.758 0.750 0.618
    1250.455 0.432 0.444 0.312
    62.50.307 0.301 0.304 0.172
    31.250.228 0.226 0.227 0.095
    00.131 0.133 0.132
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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