Dog Interleukin-8,IL-8 ELISA Kit
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中文名稱:犬白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
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貨號:CSB-E08052c
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:IL-8(Interleukin-8),也稱為中性粒細(xì)胞趨化因子1(Neutrophil chemotactic factor 1,NCF-1),是一種由多種細(xì)胞類型分泌的趨化因子,主要作用是吸引和激活白細(xì)胞(尤其是中性粒細(xì)胞)在炎癥部位定向運(yùn)動。IL-8通過結(jié)合兩種不同的趨化因子受體CXCR1和CXCR2來介導(dǎo)其生物學(xué)效應(yīng)。在機(jī)體免疫和炎癥反應(yīng)中,IL-8的表達(dá)水平顯著升高,因此被廣泛應(yīng)用于炎癥和免疫疾病的研究和診斷。此外,IL-8還參與了腫瘤的生長、轉(zhuǎn)移和血管生成等生物學(xué)過程。華美生物所提供的Dog Interleukin-8,IL-8 ELISA Kit屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測狗血清、血漿、細(xì)胞培養(yǎng)上清、組織勻漿樣本中的IL8,其靈敏度為0.146 ng/mL,檢測范圍為0.312 ng/mL-20 ng/mL。
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別名:CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.146 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 89-97 1:2 Average % 99 Range % 95-105 1:4 Average % 88 Range % 85-93 1:8 Average % 90 Range % 86-97 -
回收率:
The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 86-94 EDTA plasma (n=4) 88 85-95 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 3.221 3.295 3.258 3.157 10 2.523 2.711 2.617 2.516 5 1.791 1.930 1.861 1.760 2.5 1.035 1.045 1.040 0.939 1.25 0.466 0.485 0.476 0.375 0.625 0.249 0.259 0.254 0.153 0.312 0.157 0.167 0.162 0.061 0 0.103 0.099 0.101 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Synergistic Action of IL-8 and Bone Marrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors Dong Suk Yoon. et al,Tissue Engineering Part A.,2016
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:IL8,全稱為白細(xì)胞介素8,是一種由免疫細(xì)胞產(chǎn)生的炎癥介質(zhì)。IL8通過結(jié)合CXCR1和CXCR2等受體調(diào)節(jié)免疫細(xì)胞的趨化和活化,參與多種炎癥反應(yīng)的發(fā)生和發(fā)展。近年來的研究表明,IL8在腫瘤的發(fā)生和轉(zhuǎn)移中發(fā)揮重要作用。例如,IL8的高表達(dá)與乳腺癌、結(jié)直腸癌等多種腫瘤的不良預(yù)后相關(guān)。
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功能:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
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基因功能參考文獻(xiàn):
- Analysis of ROC suggests the use of serum levels of MCP-1 and IL-7 as predictors of the occurrence of complications with an AUC of 0.906 and 0.896 respectively and linear combinations of MCP-1, KC-Like, IL-7 and GM-CSF with values up to AUC = 0.983 PMID: 29304171
- These observations indicate that the individual activation of ERK2 and JNK1 pathways contributes to TNF-alpha-induced IL-8 expression in synovial fibroblasts. PMID: 28806729
- data suggest that CCL2 acts as a PMNs chemotactic factor as well as CXCL8, and both CCL2 and CXCL8 facilitate the infiltration of PMNs into the joints of idiopathic polyarthritis PMID: 27863550
- The study findings suggest that both CCL2 and CXCL8 are involved in the pathogenesis of canine idiopathic pulmonary fibrosis. PMID: 26231926
- High CXCL8 blood concentrations might be related to the breed predisposition of the West Highland white terrier for canine idiopathic pulmonary fibrosis. PMID: 26267090
- IL-8-positive macrophages were significantly increased in large polyps compared to controls PMID: 24148828
- hemangiosarcoma derived IL-8 may play a role in tumor development by modulating the tumor microenvironment PMID: 24582862
- Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
- serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
- Sixty-one percent (28/46) of the samples from canine stifle osteoarthritis had IL-8 mRNA expression in contrast to 4% (1/24) in the control stifle joints PMID: 16102844
- Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
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亞細(xì)胞定位:Secreted.
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蛋白家族:Intercrine alpha (chemokine CxC) family
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數(shù)據(jù)庫鏈接:
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