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Chicken growth hormone,GH ELISA KIT

  • 中文名稱：
    雞生長激素(GH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09866Ch
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Chicken growth hormone (GH) ELISA Kit was designed for the quantitative measurement of Chicken growth hormone (GH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 625 pg/mL-10000 pg/mL and the sensitivity is 625 pg/mL.

  • 別名：
    GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
    GH
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    625 pg/mL-10000 pg/mL
  • 靈敏度：
    625 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Developmental Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-98  
    1:2 Average % 99  
    Range % 92-105  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 90  
    Range % 83-98  
  • 回收率：
    The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 88-96  
    EDTA plasma (n=4) 94 91-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    10000 0.154 0.145 0.150    
    5000 0.261 0.244 0.253    
    2500 0.538 0.534 0.536    
    1250 1.113 1.139 1.126    
    625 2.356 2.278 2.317    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Growth hormone plays an important role in growth control.
  • 基因功能參考文獻(xiàn)：
    1. This is the first study to demonstrate that single nucleotide polymorphisms in GHSR can increase appetite, growth traits, expression and level of GHRL, suggesting a hunger signal role for endogenous GHRL. PMID: 27591070
    2. There is a negative glucocorticoid response-like element located in the +591/+609 region within the first intron of chicken growth hormone (cGH), which is essential for the down-regulation of cGH expression. PMID: 27834851
    3. It is shown that in all examined chicken populations the growth hormone gene is polymorphic. PMID: 26841491
    4. The molecular mechanisms underlying glucocorticoid recruitment of somatotrophs during embryogenesis were studied; these provide insight into glucocorticoid-induced developmental changes in other tissues and cell types. PMID: 25560830
    5. T185G growth hormone polymorphism was significantly associated with the egg production traits in chickens. PMID: 25062422
    6. GHRH stimulates testicular GH secretion in an autocrine or paracrine manner. PMID: 24508498
    7. The results indicate that high frequency of I, C and A alleles corresponds to the egg-laying chicken lines. PMID: 23285753
    8. A 1727-bp fragment of 5'-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. PMID: 15229358
    9. small chicken GH expression and translation occurs in ocular tissues of chick embryos, in which its localization in the neural retina and the optic nerve head is distinct from that of the full-length protein PMID: 17008400
    10. The identification of 3 restriction enzyme polymorphic sites were detected in the growth hormone gene is reported. PMID: 17495076
    11. Results showed that there was no significant difference between dwarf and normal chickens in GH expression. PMID: 17681929
    12. results demonstrate presence of growth hormone and its receptors in the ovarian stroma with cortical follicles & in the wall of small and large follicles of the ovary during sexual maturation PMID: 18335240
    13. Positive somatotroph responses to corticosterone (CORT) are lost during chicken embryonic development and that glucocorticoid receptors are the primary regulator of CORT-induced growth hormone secretion. PMID: 19157766

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：