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中文名稱:雞熱休克蛋白90(HSP-90)酶聯(lián)免疫試劑盒
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貨號:CSB-E12873C
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Chicken HSP90AA1 ELISA Kit was designed for the quantitative measurement of Chicken HSP90AA1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
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別名:HSP90AA1 ELISA Kit; HSPCAHeat shock protein HSP 90-alpha ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Gallus gallus (Chicken)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken HSP-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 85-103 1:2 Average % 89 Range % 85-96 1:4 Average % 93 Range % 83-99 1:8 Average % 95 Range % 89-98 -
回收率:
The recovery of chicken HSP-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-101 EDTA plasma (n=4) 95 87-99 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.428 2.388 2.408 2.303 50 1.994 1.935 1.965 1.860 25 1.362 1.334 1.348 1.243 12.5 0.830 0.868 0.849 0.744 6.25 0.618 0.585 0.602 0.497 3.12 0.345 0.342 0.344 0.239 1.56 0.251 0.262 0.257 0.152 0 0.103 0.107 0.105 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- EFFECT OF EUCALYPTUS LEAVES AND ITS SUPPLEMENTATION WITH DIET ON BROILER PERFORMANCE, MICROBIAL AND PHYSIOLOGICAL STATUES TO ALLEVIATE COLD STRESS M. AG. Mustafa, et al,Iraqi journal of agricultural sciences,2019
- HSP90 gene expression induced by aspirin is associated with damage remission in a chicken myocardial cell culture exposed to heat stress. Zhang X.et al,Br Poult Sci.,2016
- The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells. Zhang XH.et al,J Vet Sci.,2016
相關產(chǎn)品
靶點詳情
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功能:Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from Hsp90 which acquires an open conformation for the next cycle.
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亞細胞定位:Cytoplasm. Melanosome.
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蛋白家族:Heat shock protein 90 family
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數(shù)據(jù)庫鏈接:
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