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Canine calprotectin (CALP) ELISA kit

  • 中文名稱：
    犬鈣衛(wèi)蛋白(CALP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EQ013485DO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Dog CALP ELISA Kit was designed for the quantitative measurement of Dog CALP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

  • 縮寫：
    CALP
  • 種屬：
    Canine
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 86    
    Range % 81-92    
    1:2 Average % 102    
    Range % 97-107    
    1:4 Average % 96    
    Range % 85-99    
    1:8 Average % 103    
    Range % 96-107    
  • 回收率：
    The recovery of canine CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 94 89-98    
    EDTA plasma (n=4) 102 97-106    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    400 1.816 1.921 1.869 1.723    
    200 1.448 1.512 1.480 1.334    
    100 1.030 1.078 1.054 0.908    
    50 0.758 0.733 0.746 0.600    
    25 0.476 0.466 0.471 0.325    
    12.5 0.348 0.332 0.340 0.194    
    6.25 0.275 0.268 0.272 0.126    
    0 0.145 0.146 0.146 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

Would you provide the information:
1) antibody clonality _ are capture and detection antibodies monoclonal or polyclonal?
2) antibody's target immunogen _ to which immunogen have detection and capture antibodies of the kit been raised? was it of human or canine origin? was it a native or recombinant protein? was it a full length protein or a fragment?
3) proteins detected in the samples by the antibodies _ do the
antibodies of the kit detect only the S100A8/S100A9 heterocomplex or do they also detect S100A8 and S100A9 proteins separately (as monomers or homodimers)?
4) standard protein _ is the protein used as the analytical standard of the kit of canine or human origin? is it a native or recombinant protein? is it a full length protein or a fragment?

A:
Thanks for your inquiry!CSB-EQ013485DO detects CALP(Calprotectin is a complex of the mammalian proteins S100A8 and S100A9)
The kit uses 2 antibodies, the Coated anti. and Detect anti. are both Goat polyclonal antibody, which are designed for the information of the links
https://www.ncbi.nlm.nih.gov/protein/XP_013970901.1 and https://www.ncbi.nlm.nih.gov/protein/NP_001139616.1 separately.
The immunogen is 293 cell-derived recombinant protein.
The standard is the purified crude product of the natural sample,but so sorry the specific source information can not be disclosed.