Bovine interleukin 4,IL-4 ELISA Kit
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中文名稱:牛白介素4(IL-4)酶聯(lián)免疫試劑盒
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貨號:CSB-E12898B
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規(guī)格:96T/48T
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價格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Bovine IL4 ELISA Kit was designed for the quantitative measurement of Bovine IL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .
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別名:IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Bos taurus (Bovine)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:12.5 pg/mL-800 pg/mL
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靈敏度:3.12 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 89-99 1:2 Average % 97 Range % 92-103 1:4 Average % 90 Range % 85-94 1:8 Average % 84 Range % 80-88 -
回收率:
The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 89 84-93 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 800 1.993 1.912 1.953 1.835 400 1.576 1.616 1.596 1.478 200 1.304 1.354 1.329 1.211 100 0.939 0.962 0.951 0.833 50 0.624 0.641 0.633 0.515 25 0.441 0.419 0.430 0.312 12.5 0.283 0.279 0.281 0.163 0 0.119 0.117 0.118 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea ZL Fu,Journal of dairy science,2024
- Antimicrobial Resistance of Staphylococcus aureus and Coagulase?negative Staphylococci from Bovine Mastitis Milk with Detection of Interleukins in Milk and Serum of Infected Cows A Shehab,Journal of Advanced Veterinary Research,2023
- The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
- Hematological, immunological, and polyamines alterations in the concomitant occurrence of Fasciola gigantica and hepatic leiomyoma in cattle S Shahvazi,Veterinary Parasitology,2021
- Attenuation of bovine herpesvirus type 1 by deletion of its glycoprotein G and tk genes and protection against virulent viral challenge Minmin Zhang et al,Vaccine,2011
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
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基因功能參考文獻(xiàn):
- These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving. PMID: 23814356
- IL-4 may play a role in coordinating the adrenal response to inflammatory stress. PMID: 18055157
- T helper (Th) type 2 cell cytokine IL-4 modulates airway contraction by secreting matrix metalloproteinase-1 from the smooth muscle cells via phosphatidylinositol 3-kinase activation and changing cell-to-matrix interactions. PMID: 18322231
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亞細(xì)胞定位:Secreted.
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蛋白家族:IL-4/IL-13 family
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數(shù)據(jù)庫鏈接:
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