Bovine gonadotropin-releasing hormone(GnRH)ELISA Kit
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中文名稱:牛促性腺激素釋放激素(GnRH)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E15857B
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Bovine GnRH ELISA Kit was designed for the quantitative measurement of Bovine GnRH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 100 pg/mL-4000 pg/mL and the sensitivity is 100 pg/mL .
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縮寫(xiě):GnRH
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Uniprot No.:
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種屬:Bos taurus (Bovine)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:100 pg/mL-4000 pg/mL
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靈敏度:100 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 83-95 1:2 Average % 95 Range % 91-101 1:4 Average % 95 Range % 89-101 1:8 Average % 90 Range % 85-98 -
回收率:
The recovery of bovine GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 97 94-105 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 0 2.513 2.527 2.520 100 1.042 1.032 1.037 300 0.522 0.510 0.516 920 0.380 0.364 0.372 2000 0.261 0.246 0.254 4000 0.132 0.134 0.133 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Biosynthesis of gonadotropin-releasing hormone (GnRH) and GnRH receptor (GnRHR) in hypothalamic?pituitary unit of anoestrous and cyclic ewes M.O. Ciechanowska.et al,Canadian Journal of Physiology and Pharmacology,2016
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