Bovine Interleukin 6,IL-6 ELISA Kit
-
中文名稱:牛白介素6(IL-6)酶聯(lián)免疫試劑盒
-
貨號(hào):CSB-E12899B
-
規(guī)格:96T/48T
-
價(jià)格:¥3800/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:IL6 (Interleukin-6) 是一種細(xì)胞因子,在免疫反應(yīng)、炎癥反應(yīng)、細(xì)胞增殖和分化等生物過程中發(fā)揮重要作用。它能夠刺激機(jī)體免疫細(xì)胞的生長(zhǎng)、增殖和分化,并調(diào)節(jié)機(jī)體的免疫反應(yīng),其高含量與多種炎癥疾病的發(fā)生和發(fā)展相關(guān)。華美生物所提供的Bovine Interleukin 6,IL-6 ELISA Kit屬于ELISA檢測(cè)試劑盒,采用競(jìng)爭(zhēng)法定量檢測(cè)牛血清、血漿、組織勻漿樣本中的IL6,其靈敏度為2.5 pg/mL,檢測(cè)范圍為5 pg/mL-1000 pg/mL。
-
別名:IL6 Interleukin-6 ELISA Kit; IL-6 ELISA Kit
-
縮寫:
-
Uniprot No.:
-
種屬:Bos taurus (Bovine)
-
樣本類型:serum, plasma, tissue homogenates
-
檢測(cè)范圍:5 pg/mL-1000 pg/mL
-
靈敏度:2.5 pg/mL
-
反應(yīng)時(shí)間:1-5h
-
樣本體積:50-100ul
-
檢測(cè)波長(zhǎng):450 nm
-
研究領(lǐng)域:Immunology
-
測(cè)定原理:quantitative
-
測(cè)定方法:Competitive
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess.
-
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 90-97 1:2 Average % 95 Range % 91-99 1:4 Average % 95 Range % 88-101 1:8 Average % 95 Range % 90-100 -
回收率:
The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 94-101 EDTA plasma (n=4) 96 92-103 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 1000 0.071 0.075 0.073 250 0.192 0.205 0.199 80 0.421 0.451 0.436 20 0.876 0.897 0.887 5 1.345 1.316 1.331 0 2.116 2.215 2.166 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
引用文獻(xiàn)
- Effects of Lonicera japonica Extract with Different Contents of Chlorogenic Acid on Lactation Performance, Serum Parameters, and Rumen Fermentation in Heat-Stressed Holstein High-Yielding Dairy Cows F Ma,Animals,2024
- Associations of Neonatal Dairy Calf Faecal Microbiota with Inflammatory Markers and Future Performance M Loch,/,/
- Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea ZL Fu,Journal of dairy science,2024
- Cellular uptake, intracellular behavior, and acute/sub-acute cytotoxicity of a PEG-modified quantum dot with promising in-vivo biomedical applications Q Cheng,Heliyon,2023
- Concentrations of serum amyloid A, haptoglobin and some cytokines in calves with Cryptosporidiosis in the pre-and post-treatment stage K Mustafa?,Journal of Advances in VetBio Science and Techniques,2023
- Local and Systemic Inflammation in Finnish Dairy Cows with Digital Dermatitis YS Wang,Animals,2024
- Comparative Study of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results H Hu,Microorganisms,2024
- Antimicrobial Resistance of Staphylococcus aureus and Coagulase?negative Staphylococci from Bovine Mastitis Milk with Detection of Interleukins in Milk and Serum of Infected Cows A Shehab,Journal of Advanced Veterinary Research,2023
- Associations of neonatal acute phase response with first lactation performance in dairy cows M Loch,Journal of dairy science,2023
- The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
- Dimethyl itaconic acid improves viability and steroidogenesis and suppresses cytokine production in LPS-treated bovine ovarian granulosa cells by regulating TLR4/nfkβ, NLRP3, JNK signaling pathways MR Tabandeh,Research in veterinary science,2022
- Faecal microbiota in two-week-old female dairy calves during acute cryptosporidiosis outbreak–Association with systemic inflammatory response E Dorbek-Kolin,Research in Veterinary Science,2022
- Effect of colostrum on the acute-phase response in neonatal dairy calves K Peetsalu,Journal of dairy science,2022
- Upregulation of Matrix Metalloproteinase-9 Protects against Sepsis-Induced Acute Lung Injury via Promoting the Release of Soluble Receptor for Advanced Glycation End Products H Zhang,Oxidative Medicine and Cellular Longevity,2021
- Effect of non-encapsulated and encapsulated active dried yeast on blood cell count, blood metabolites and immune response of finishing beef heifers P Jiao,Canadian Journal of Animal Science ja,2021
- Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line E Gugliandolo,Veterinary Science,2020
- Haemato-chemical and immune variations in Holstein cows at different stages of lactation, parity, and age SB Kim,CAAS Agricultural Journals,2020
- Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,Animal Feed Science and Technology,2020
- Protective Effects of Astaxanthin on Lipopolysaccharide-Induced Inflammation in Bovine Endometrial Epithelial Cells Wan F C, et al,Biology of Reproduction,2019
- Caffeic Acid Prevented LPS-Induced Injury of Primary Bovine Mammary Epithelial Cells through Inhibiting NF-κB and MAPK Activation Mingjiang Liu,Mediators of Inflammation,2019
- Supplementation with fat-coated rumen-protected glucose during the transition period enhances milk production and influences blood biochemical parameters of liver function and inflammation in dairy cows X.P.Li,Animal Feed Science and Technology,2019
- Immune Responses of Cattle to Theileria orientalis Infection and Seasonal Change Changyong Choe, et al,International Journal of Applied Research in Veterinary Medicine,2018
- Serum hepatokines in dairy cows: periparturient variation and changes in energy-related metabolic disorders Jianguo Wang.et al,BMC Veterinary Research,2018
- Milk fever in dairy cows is preceded by activation of innate immunity and alterations in carbohydrate metabolism prior to disease occurrence Zhang G.et al,Res Vet Sci,2017
- Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows Ning LT.et al,J Anim Physiol Anim Nutr ,2017
- Acute-phase responses in cattle infected with hydatid cysts and microbial agents. Sevimli A.et al,J Helminthol.,2015
- Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis Kabu M.et al,Veterinarni Medicina,2016
- Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,2016
- Kinetics of pro-inflammatory cytokines, interleukin-10, and virus neutralising antibodies during acute ephemeral fever virus infections in Brahman cattle Barigye R. et al,Vet Immunol Immunopathol,2015
- Contrasting Cellularity and Fatty Acid Composition in Fat Depots from Alentejana and Barrosã Bovine Breeds Fed High and Low Forage Diets /,Int J Biol Sci,2012
- A small scale study on the effects of oral administration of the ß-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves Hirofumi Uchiyama et al,BMC Research Notes,2012
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
最新研究進(jìn)展:IL6是一種白細(xì)胞介素,具有調(diào)節(jié)免疫反應(yīng)和炎癥反應(yīng)的作用。最新研究表明,IL6在心血管疾病、惡性腫瘤和神經(jīng)系統(tǒng)疾病等多種疾病的發(fā)生和發(fā)展中發(fā)揮重要作用。研究還發(fā)現(xiàn),針對(duì)IL6的治療方法在臨床實(shí)踐中取得了很好的療效,可以有效減輕疾病的癥狀和改善患者的生活質(zhì)量。
-
功能:Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
-
基因功能參考文獻(xiàn):
- STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. PMID: 26813342
- when a confluent endometrial epithelial cell barrier is faced with infection and damage, chemokines attract immune cells to the uterine lumen, but IL6 is solely secreted apically to ensure immune cells are only exposed to IL6 once they reach the lumen. PMID: 25740541
- The results revealed that the peak expression of IL6 and 21 was on DPV 28 which correlated well with the FMDV antibody titer and plummeted to the prevaccination titer level by 60 DPV. PMID: 24555796
- Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
- Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
- The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
- results show for the first time that interleukin-6 (IL6), in the presence of its soluble receptor (sIL-6R), induces activation of JAK1, JAK2, and STAT1/STAT3 proteins in bovine articular chondrocytes. PMID: 12419823
- Mechanical injury potentiates the catabolic effects of TNFalpha and IL-6/sIL-6R in causing proteoglycan degradation in human and bovine cartilage. PMID: 19790045
顯示更多
收起更多
-
亞細(xì)胞定位:Secreted.
-
蛋白家族:IL-6 superfamily
-
數(shù)據(jù)庫(kù)鏈接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-