Bovine Interleukin 2,IL-2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

This Bovine IL2 ELISA Kit was designed for the quantitative measurement of Bovine IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
別名：

IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
縮寫：

IL2
Uniprot No.：

P05016
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-90
1:2	Average %	100
	Range %	97-103
1:4	Average %	101
	Range %	98-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能參考文獻(xiàn)：
1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-2 family
數(shù)據(jù)庫鏈接：

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341