Bovine Interferon γ,IFN-γ ELISA Kit
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中文名稱(chēng):牛γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09812b
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Bovine IFNG ELISA Kit was designed for the quantitative measurement of Bovine IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
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別名:IFNG ELISA Kit; Interferon gamma ELISA Kit; BoIFNG ELISA Kit; IFN-gamma ELISA Kit
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縮寫(xiě):
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Uniprot No.:
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種屬:Bos taurus (Bovine)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.8 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-95 1:2 Average % 102 Range % 99-105 1:4 Average % 94 Range % 89-97 1:8 Average % 97 Range % 93-101 -
回收率:
The recovery of bovine IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-96 EDTA plasma (n=4) 95 92-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 1.974 1.882 1.928 1.785 1000 1.290 1.196 1.243 1.100 500 0.818 0.807 0.813 0.670 250 0.519 0.502 0.511 0.368 125 0.324 0.333 0.329 0.186 62.5 0.268 0.280 0.274 0.131 31.25 0.212 0.221 0.217 0.074 0 0.142 0.144 0.143 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Unleashing lactoferrin's antidepressant potential through the PI3K/Akt/mTOR pathway in chronic restraint stress rats HH Ahmed,Food & function,2023
- Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea ZL Fu,Journal of dairy science,2024
- The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
- Association of the MACROD2 rs6110695 A>G polymorphism with an increasing WBC count in a Korean population J Yang,Immunity, inflammation and disease,2022
- The effect of staphylococcal mastitis including resistant strains on serum procalcitonin, neopterin, acute phase response and stress biomarkers in Holstein dairy cows W El-Deeb,PeerJ,2021
- Protective immune response against P32 oncogenic peptide-pulsed PBMCs in mouse models of breast cancer M Dehghan-Manshadi,Environmental Science and Pollution Research,2021
- Haemato-chemical and immune variations in Holstein cows at different stages of lactation, parity, and age SB Kim,CAAS Agricultural Journals,2020
- Immune Responses of Cattle to Theileria orientalis Infection and Seasonal Change Changyong Choe, et al,International Journal of Applied Research in Veterinary Medicine,2018
- Kinetics of Interferon gamma and Interleukin-21 response following foot and mouth disease virus infection Ajay Kumar Sharma .et al,Microbial Pathogenesis,2018
- Dietary effects of lutein-fortified chlorella on milk components of Holstein cows. Jeon JY.et al,Springerplus.,2016
- Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,2016
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
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基因功能參考文獻(xiàn):
- Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-gamma production via PD-1/PD-L1 blockade in bovine mycoplasmosis. PMID: 28544524
- The expression of multiple toll-like receptors, interferon-gamma, and interleukin-12 (IL-12) in cattle with low and high proviral loads of bovine leukemia virus are reported. PMID: 27473994
- Diet-driven interferon-gamma enhances malignant transformation of primary bovine mammary epithelial cells through nutrient sensor GCN2-activated autophagy. PMID: 26962688
- Data suggest that luteolytic factors (such as IFNG, tumor necrosis factor alpha, and PGF2a) control expression of MMP1, other matrix metalloproteinases, and tissue inhibitors of metalloproteinase in cultured luteal cells. PMID: 25924700
- These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
- These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving. PMID: 23814356
- Genetic characterization of IFNG gene was done in resistant and susceptible animals of Sahiwal cattle (n = 95) and Friesian (n = 92). PMID: 21960011
- Data suggest that activation of gammadelta T cells to IFN-gamma production, NK cell-like killing plays a pivotal role in controlling virus infection. PMID: 21383249
- The differential expression levels of IFN-gamma mRNA between cattle and buffalo could be due to a conserved 4 base (GTCT) deletion in the promoter region of buffalo. PMID: 20541234
- SNPs in IFNG, IFNGR1 and R2, IL22, and IL22RA1 were analyzed for an association to Estimated breeding values for somatic cell score in Canadian Holstein bulls; no significant associations were found. PMID: 21328101
- Nuclear localization sequence of bovine gamma-interferon provides translocation of recombinant protein to yeast Pichia pastoris cell nucleus PMID: 21268843
- The upregulation of TNFRI mRNA expression by IFNG suggests that TNF and IFNG synergistically affect the death of luteal endothelial cells resulting in acute luteolysis PMID: 20562522
- Data demonstrated that the monoclonal antibodies secreted by the four hybridoma cell lines could react specifically to the recombinant BovIFN-gamma. PMID: 17366886
- The entire bovine IFN-gamma gene (BoIFNG) and 2605 bp of its promoter DNA were sequenced. A comprehensive survey for polymorphisms in the bovine IFN-gamma gene reveals a highly polymorphic intronic DNA sequence allowing improved genotyping of Bovinae. PMID: 12396714
- Bovine interferon gamma demonstrates no effect on P-glycoprotein transport activity in human Caco-2 intestinal epithelial cells with rhodamine 123 as susbstrate. PMID: 15124210
- In giant cell arteritis, IFN-gamma functional polymorphisms are associated with clinical manifestations of severity rather than susceptibility to this vasculitis. PMID: 15675129
- The majority of patients with gad-enhancing lesions showed PLP/IFN gamma and MBP/IFN gamma recurrent burst responses PMID: 15957503
- A polymorphism within the interferon gamma gene is a risk factor in severe acute respiratory syndrome susceptibility. PMID: 16672072
- Intradermal sensitization of cows in the subclinical stage of M. paratuberculosis infection will upregulate expression of interferon gamma, enhancing the sensitivity of this assay. PMID: 17459831
- This study compared the protective immune responses to bovine tuberculosis induced in cattle vaccinated with BCG Danish with those induced by BCG Pasteur. PMID: 17524495
- Lipopolysaccharide stimulation up-regulates the secretion of cytokines by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
- Taken together, these results support the hypothesis of an inactivating phosphorylation of nNOS by IFNgamma, via JAK/STAT, in bovine chromaffin cells. PMID: 19141072
- A tendency toward statistical significance for the effect of polymorphisms in the odds of infection in cattle was not found for alleles IFNG. PMID: 19525022
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亞細(xì)胞定位:Secreted.
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蛋白家族:Type II (or gamma) interferon family
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組織特異性:Released primarily from activated T lymphocytes.
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