This Bovine APOA1 ELISA Kit was designed for the quantitative measurement of Bovine APOA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL .
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1000
Average %
90
Range %
87-94
1:2000
Average %
96
Range %
91-100
1:4000
Average %
93
Range %
87-97
1:8000
Average %
100
Range %
96-103
回收率:
The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
84
82-87
EDTA plasma (n=4)
89
85-96
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能參考文獻(xiàn):
the model of a two-step process for the transendothelial transport of apoA-I in which apoA-I is initially lipidated by ABCA1 and then further processed by ABCA1-independent mechanisms. PMID: 21209084
亞細(xì)胞定位:
Secreted.
蛋白家族:
Apolipoprotein A1/A4/E family
組織特異性:
Major protein of plasma HDL, also found in chylomicrons.