LTA

LTA基因編碼TNF-β蛋白（腫瘤壞死因子β），也稱(chēng)為淋巴毒素α（Lymphotoxin Alpha，LT-α），屬于TNSF超家族一員。根據(jù)TNF來(lái)源的不同，人們將單核/巨噬細(xì)胞所產(chǎn)生的TNF稱(chēng)腫瘤壞死因子α；把T細(xì)胞產(chǎn)生的TNF稱(chēng)為腫瘤壞死因子（TNF-β）。TNF-β有膜結(jié)合和可溶性?xún)煞N形式，分泌到細(xì)胞外的可溶性形式稱(chēng)為L(zhǎng)T-α。LT-α主要由活化的CD4+CD8+T細(xì)胞、NK細(xì)胞、B淋巴母細(xì)胞樣細(xì)胞、骨髓瘤細(xì)胞等產(chǎn)生。LT-α在細(xì)菌的代謝和致病過(guò)程中發(fā)揮重要的作用。研究證實(shí)在感染結(jié)核分支桿菌后，遺傳性LT-a缺陷的小鼠比LT-a正常小鼠屯集更多的細(xì)菌負(fù)荷量。TNF-β除了介導(dǎo)許多炎癥、免疫刺激和抗病毒應(yīng)答。在發(fā)育階段，TNF-β還參與二級(jí)淋巴器官的形成，并在凋亡中發(fā)揮作用。在TNF-β敲除小鼠中，所有派伊爾氏淋巴集結(jié)和淋巴結(jié)都無(wú)法發(fā)育，這表明了TNF-β在免疫系統(tǒng)發(fā)育中的重要性。

Recombinant Human LTA 活性蛋白實(shí)驗(yàn)驗(yàn)證數(shù)據(jù)

High Purity Validated
by SDS-PAGE

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 μg/ml can bind human TNFRSF1B (CSB-MP023978HU2), the EC₅₀ is 1.632-2.699 ng/ml.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 μg/ml can bind human TNFR1(CSB-MP023977HU1), the EC₅₀ of human LTA protein is 4.409-6.797 ng/ml.