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Recombinant Mouse Aquaporin-2 (Aqp2)

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  • 中文名稱：
  • 貨號：
    CSB-CF001962MO
  • 規(guī)格：
    ￥9720
  • 促銷：
    現(xiàn)貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Aqp2
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length
  • 來源：
    in vitro E.coli expression system
  • 分子量：
    30.5 kDa
  • 表達區(qū)域：
    1-271aa
  • 氨基酸序列
    MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGTLVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRSDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAVIGSLLYNYLLFPSTKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis.
  • 基因功能參考文獻：
    1. Vasopressin induces apical actin skeleton relaxation to facilitate AQP2 insertion to the apical plasma membrane. PMID: 24085853
    2. calcineurin is a key regulator of Wnt5a-induced AQP2 activation without affecting intracellular cAMP level and PKA activity. PMID: 27892464
    3. vasopressin-mediated transcriptional regulation is highly selective for Aqp2 PMID: 27725713
    4. Data suggest that membrane-proximal signaling complexes constrained by AKAP220 impact the actin barrier dynamics and AQP2 trafficking to ensure water homeostasis. PMID: 27402760
    5. These studies indicate that manipulation of the cholesterol content of the basolateral plasma membrane interferes with AQP2 posttranslational modification and subsequently regulated apical plasma membrane targeting of AQP2. PMID: 29103957
    6. It was proposed that FXYD1 plays a role in regulating AQP2 retention in apical membrane, and that this involves transfers between raft-like membrane domains in endosomes and plasma membranes. PMID: 29155857
    7. Both NFATc3 knock-out mice and ILK conditional-knockdown mice (cKD-ILK) display symptoms of NDI (polyuria and reduced AQP2 expression). PMID: 28736155
    8. aquaporin-2 regulates serine/threonine phosphatases in renal collecting duct PMID: 27784696
    9. Ativation of LXRbeta increases AQP2 protein levels in the renal collecting ducts via a posttranscriptional mechanism top regulate water electrolyte balance. PMID: 28052875
    10. PDZ domain-containing protein Sipa1l1 (signal-induced proliferation-associated 1 like 1) binds to the cytoplasmic PDZ-ligand motif of aquaporin-2 and accelerates its endocytosis in the absence of vasopressin PMID: 28336531
    11. Conditional inactivation of Elf5 in the developing collecting ducts results in a small but significant reduction in the expression levels of Aqp2 and Avpr2 genes. PMID: 28215940
    12. These results provide the direct evidence that the Ser-261 dephosphorylation is involved in the pS256- and pS269-related AQP2 regulation. PMID: 27889609
    13. analysis of phosphorylation-dependent interactions of AQP2 with 14-3-3theta; and -zeta PMID: 26645691
    14. the activation of CaSR in the collecting duct prevents the cyclic AMP-dependent increase in AQP2-phosphorylation at S256 and water permeability, counteracting the short-term vasopressin response. PMID: 25977473
    15. Functional photoconvertible chimeric AQP-2 was successfully expressed in mpkCCD cells, in which forskolin induced apical trafficking and accumulation of chimeric AQP-2. PMID: 25998606
    16. Suggest ERalpha in mediates the inhibitory effect of estradiol on AQP2 expression in collecting ducts. PMID: 26062878
    17. Data suggest that the ability of prostaglandin E2 receptor EP4 to promote aquaporin 2 (AQP2) membrane targeting and increase AQP2 abundance makes it a therapeutic target for the treatment of congenital diabetes insipidus. PMID: 26100911
    18. Odontoblast-lineage cell line have high-cell viability under xylitol-induced hypertonic stress, which may be associated with TRPV1 and AQP2 expressions. PMID: 25372661
    19. The direct renin inhibitor aliskiren increased water channel AQP2 expression in obstructed kidneys of UUO mice, at least partially by preventing NLRP3 inflammasome activation in association with ureteral obstruction. PMID: 25694485
    20. role for PKA signaling in both short- and long-term regulation of AQP2, characterizing a novel mouse model of diabetes insipidus PMID: 25587115
    21. tankyrase likely to play an important role in vasopressin-induced AQP2 upregulation via beta-catenin-mediated transcription in the kidney collecting duct cells PMID: 25520007
    22. Studied the effect of the total tannins extract of rhubarb on expression od aquaporin 2 and auqaporin 3 in diarrhoea mice. PMID: 25215286
    23. increased expression in inappropriate antidiuresis syndrome PMID: 24700872
    24. AQP2 is subjected to S-glutathionylation, which is modulated by reactive oxygen species production. PMID: 25112872
    25. Tubular AQP2 was decreased in expression and apical membrane presence in conditional knockout ILK mice. ILK regulates expression of AQP2 and its presence in renal tubule apical membrane. PMID: 24784577
    26. It is concluded that NaCl and urea synergistically induce the expression of UTA2 rather than AQP2 in mIMCD3 cells, and hyperosmolality probably mediates the expression of AQP2 and UTA2 through different mechanisms. PMID: 22282246
    27. GATA2 regulates the Aqp2 gene expression in collecting duct cells and contributes to the maintenance of the body water homeostasis PMID: 24636993
    28. V1aR signaling may be fundamentally important for the expression of AQP2 in the collecting ducts during control conditions and dehydration PMID: 22968856
    29. Genetic ablation of aquaporin-2 in the mouse connecting tubules results in defective renal water handling PMID: 23359673
    30. upregulated Aqp5 may contribute to polyuria, possibly by impairing Aqp2 membrane localization, in Dot1l(AC) mice and in patients with diabetic nephropathy. PMID: 23326416
    31. These data suggest that Dot1l deletion facilitates the differentiation of some alpha- and beta-intercalated cells from Aqp2-expressing progenitor cells or mature principal cells. PMID: 23308014
    32. Aquaporin 2 promotes cell migration and epithelial morphogenesis. PMID: 22859853
    33. Report beneficial effect of Poria cocos extract in water balance against in vitro hypertonic stress of renal collecting ducts. PMID: 22414475
    34. In polycystic kidney disease, structural or functional loss of cilia leads to abnormal trafficking of AQP2/V2R leading to enhanced salt and water absorption. PMID: 22205228
    35. Results indicate that Inpp5k 5-phosphatase is important for the control of the arginine vasopressin/aquaporin-2 signalling pathway and water transport in kidney collecting ducts. PMID: 21938401
    36. decreased expression of AS160 is likely to play a role in the translocation of aquaporin-2 to the plasma membrane PMID: 21511697
    37. two novel segments (-283 to -252 and -157 to -126 bp) in the AQP2-promoter mediate the hypotonicity-induced AQP2 down-regulation during vasopressin-escape. PMID: 21324903
    38. ANG II regulates AQP2 protein, trafficking, and gene expression in renal collecting duct principal cells. PMID: 21325494
    39. elevation of cAMP increased AQP2 protein levels within 30 minutes in kidney PMID: 20724536
    40. AQP2 is a renal functional molecule of vasopressin that controls urinary volume and that AQP2 in the kidney increases to alleviate dehydration due to type 2 diabetes with polyuria. PMID: 20832413
    41. Activation of the cyclic GMP pathway does not induce AQP2 expression, but it does induce AQP2 tranlocation to the apical memebrane in cortical collecting ducts. PMID: 19666909
    42. Results suggest that vasopressin-mediated membrane accumulation of AQP2 can be controlled via regulated exocytosis and endocytosis in a process that is dependent on COOH terminal phosphorylation and subsequent protein-protein interactions. PMID: 19966308
    43. NF-kappaB activation is of importance for the downregulation of AQP2 channel and vasopressin V(2) receptor expression during sepsis. NF-kappaB inhibition ameliorates sepsis-induced acute renal failure. PMID: 19828675
    44. VAMP8 mediates the regulated fusion of AQP2-positive vesicles with the plasma membrane. PMID: 19841070
    45. delineates induction and degradation mechanisms of AQP2 endogenously expressed by a renal collecting duct principal cell line PMID: 11782489
    46. AQP2 expression is regulated by aldosterone in mouse cultured renal collecting duct principal cells PMID: 12660245
    47. Jpk is a potent inducer of apoptosis through generation of reactive oxygen species and reduction of mitochondrial membrane potentials. PMID: 15033765
    48. upregulation of the UT-A2 urea transporter and the aquaporin 2 (AQP2) and AQP3 water channel transcripts but no change in other urea transporters or AQP in urea transporter B null mice PMID: 15100356
    49. These results indicate that in response to AVP AQP2 protein abundance in collecting duct principal cells is principally influenced by AQP2 mRNA content but is additionally regulated by PKA-dependent negative feedback acting on AQP2 protein degradation. PMID: 15985652
    50. The data imply that polydipsia in STR/N mice may have affected AQP2 mRNA transcription in the kidney, resulting in reduced AQP2 expression, which would contribute to a reduction in overretention of water. PMID: 16144968

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  • 亞細胞定位：
    Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 組織特異性：
    Detected in principal cells in the collecting duct in kidney medulla and cortex (at protein level). Expressed in a radial pattern from the cortex through the outer medulla into the inner medulla. Higher levels in the inner medulla.
  • 數(shù)據(jù)庫鏈接：