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  4. Recombinant Human Potassium channel subfamily K member 3 (KCNK3)

Recombinant Human Potassium channel subfamily K member 3 (KCNK3)

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  • 中文名稱：
    人KCNK3重組蛋白
  • 貨號：
    CSB-CF012071HU
  • 規(guī)格：
    ￥9720
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    KCNK3
  • Uniprot No.：
  • 別名：
    KCNK3; TASK; TASK1; Potassium channel subfamily K member 3; Acid-sensitive potassium channel protein TASK-1; TWIK-related acid-sensitive K(+ channel 1; Two pore potassium channel KT3.1; Two pore K(+ channel KT3.1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    in vitro E.coli expression system
  • 分子量：
    50.5 kDa
  • 表達區(qū)域：
    1-394aa
  • 氨基酸序列
    MKRQNVRTLALIVCTFTYLLVGAAVFDALESEPELIERQRLELRQQELRARYNLSQGGYEELERVVLRLKPHKAGVQWRFAGSFYFAITVITTIGYGHAAPSTDGGKVFCMFYALLGIPLTLVMFQSLGERINTLVRYLLHRAKKGLGMRRADVSMANMVLIGFFSCISTLCIGAAAFSHYEHWTFFQAYYYCFITLTTIGFGDYVALQKDQALQTQPQYVAFSFVYILTGLTVIGAFLNLVVLRFMTMNAEDEKRDAEHRALLTRNGQAGGGGGGGSAHTTDTASSTAAAGGGGFRNVYAEVLHFQSMCSCLWYKSREKLQYSIPMIIPRDLSTSDTCVEQSHSSPGGGGRYSDTPSRRCLCSGAPRSAISSVSTGLHSLSTFRGLMKRRSSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    This recombinant HumanKCNK3 protein is an in vitro E.coli (cell-free) expressed Full Length protein. Its purity is 85%+ determined by SDS-PAGE. Cell-free protein expression is the in vitro synthesis of a protein using translation-compatible extracts of whole cells. In principle, whole-cell extracts contain all the macromolecules and components needed for transcription, translation, and even post-translational modification. These components include RNA polymerase, regulatory protein factors, transcription factors, ribosomes, and tRNA. When supplemented with cofactors, nucleotides, and the specific gene template, these extracts can synthesize proteins of interest in a few hours.

    KCNK3 is an acid-sensitive potassium channel responsible for the regulation of plasma membrane resting potential of human pulmonary artery smooth muscle cells (PASMCs). It mediates outward potassium ions current to counteract depolarization-induced calcium ions influx. Mutations in KCNK3 have been considered as a rare cause of both familial and idiopathic pulmonary arterial hypertension (PAH). The reduced KCNK3 activities caused by mutations probably lead to depolarization of the resting membrane potential, which could result in vasoconstriction and pulmonary artery remodeling. Besides, KCNK3 negatively modulates thermogenesis by inhibiting cAMP-PKA signaling.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward.
  • 基因功能參考文獻：
    1. The results of the present study suggested that miR138 promoted proliferation and suppressed mitochondrial depolarization of human pulmonary artery smooth muscle cells by targeting TASK1. PMID: 29257242
    2. Screening for mutations in the human TASK-1 gene in 155 cases of sudden infant death syndrome (SIDS) was inconclusive. Results are suggestive for an increased hypoxia-sensitivity of neonatal TASK-1(-/-) mice, however, without causing an increase in neonatal lethality. In adult female TASK-1(-/-) mice respiration was unaffected, whereas adult male TASK-1(-/-) mice showed a modified breathing pattern. PMID: 27838333
    3. Recent studies show that modulation of TASK-1 channels, either directly or indirectly by targeting their regulatory mechanisms, has the potential to control pulmonary arterial tone in humans. Furthermore, mutations in KCNK3 have been identified as a rare cause of both familial and idiopathic pulmonary arterial hypertension. [review] PMID: 29122916
    4. Sequencing of BMPR2, CAV1, and KCNK3 coding regions did not identify any pathogenic variants in these genes in infants with pulmonary hypoplasia and pulmonary hypertension. PMID: 28162765
    5. Heterozygous KCNK3 mutations in pulmonary arterial hypertension lead to variable loss of channel function via distinct mechanisms. PMID: 28889099
    6. Familial cases of Pulmonary Arterial Hypertension related to heterozygous missense variants in the KCNK3 gene (encoding the two-pore-domain potassium channel TASK-1) has been described with a prevalence of 1.3 and 3.2% in idiopathic Pulmonary Arterial Hypertension and heritable Pulmonary Arterial Hypertension, respectively. PMID: 28582316
    7. our study indicates that TASK-1 is functionally regulated by caveolin-3, possibly via association with each other on the cell surface. These results point out a novel mechanism in the regulation of TASK-1. PMID: 28648645
    8. A burden of rare variants in BMPR2 significantly contributed to the risk of pulmonary arterial hypertension. In the remaining one family, the patient carried a pathogenic variant in a member of potassium channels, KCNK3, which was the first replicative finding of channelopathy in an Asian population. PMID: 28388887
    9. The results suggested that heterodimerization of TASK1 and TALK2 provides cells with the ability to make multiple responses to a variety of physiological and pharmacological stimuli. PMID: 29016681
    10. Genetic variation in the KCNK3 gene may contribute to Blood pressure variation and less severe Hypertensive disorders in which aldosterone may be one of several causative factors PMID: 27296998
    11. Knockdown of TASK-1 by siRNA significantly enhanced apoptosis and reduced proliferation in Non-Small Cell Lung Cancer A549 cells, but not in weakly TASK-1 expressing NCI-H358 cells. PMID: 27294516
    12. The present report supports the contribution of KCNK3 mutations to the genetic etiology of Pulmonary arterial hypertension and strongly suggests that mutations in KCNK3 follow incomplete dominance with worsening of the clinical features in homozygous patients. PMID: 27649371
    13. In a cohort with idiopathic or hereditary pulmonary arterial hypertension, a possibly associated mutation was found in 11.10% of the idiopathic cases (n = 16) and in 68.18% of the hereditary cases. There were 3 mutations found in KCNK3. PMID: 27453251
    14. Functional alanine-mutagenesis screens of TASK-1 and TRAAK were used to build an in silico model of the TASK-1 cap. PMID: 26794006
    15. control of TASK-1 trafficking by COPI, kinases, phosphatases and 14-3-3 proteins is highly dynamic. PMID: 26743085
    16. KCNK3 expression and function were reduced in pulmonary artery smooth muscle cells and endothelial cells in human pulmonary arterial hypertension. PMID: 26912814
    17. TASK-1 and TASK-3 may form heterodimers in human atrial cardiomyocytes. PMID: 25655935
    18. Diacylglycerol mediates regulation of TASK1 and TASK3 potassium channels by GNAQ. PMID: 25420509
    19. Enhancement of atrium-selective K(2P)3.1 currents contributes to action potential shortening in atrial fibrillation patients. PMID: 25951834
    20. K2P3.1 and K2P9.1 undergo rapid dynamin-dependent endocytosis PMID: 23807092
    21. Syntaxin-8 regulates the endocytosis of TASK-1. PMID: 24743596
    22. A glucose-dependent role for beta-cell TASK-1 channels of limiting glucose-stimulated Deltapsip depolarization and insulin secretion, which modulates glucose homeostasis. PMID: 24932805
    23. demonstrate the functional importance of ITASK in the atrium and suggest that inactivation of TASK-1 may have diverse effects on atrial size and electrophysiological properties that can contribute to an arrhythmogenic substrate PMID: 24374141
    24. Sec61 complex reorientation of the first signal-anchor sequence of TASK-1 determines membrane topology of the other membrane spanning regions. PMID: 24015703
    25. Our study identified the association of a novel gene, KCNK3, with familial and idiopathic pulmonary arterial hypertension. PMID: 23883380
    26. TASK-1 suppresses HIV-1 replication. PMID: 23164059
    27. Ability to induce atrial fibrillation in the peri-operative period is associated with phosphorylation-dependent inhibition of TWIK protein-related acid-sensitive potassium channel 1 PMID: 23229553
    28. There were no associations between KCNK3 single nucleotide polymorphisms and blood pressure or aldosterone production. PMID: 22893713
    29. ET-1 inhibited TASK1-mediated I(KN) currents in hPASMC. PMID: 21838752
    30. I(TASK-1) contributes to the sustained outward current I(Ksus) and that I(TASK-1) is a major component of the background conductance in human atrial cardiomyocytes. PMID: 22178873
    31. TASK-1- and TASK-3-mediated currents are not affected by depletion of plasma membrane PI4,5P2 either via the voltage-activated phosphatase Ci-VSP or via chemically triggered recruitment of a PI4,5P2-5'-phosphatase. PMID: 21540350
    32. TASK-1 immunoreactivity was observed in astrocytes of temporal lobe epilepsy patients. PMID: 21710317
    33. analysis of how the specific two-pore domain potassium channel blocker A1899 defines the structure of the TASK-1 open pore PMID: 21362619
    34. cAMP-dependent protein kinase is responsible for the phosphorylation of the terminal serine in both K(2P)3.1 and K(2P)9.1 PMID: 21357689
    35. Western analysis confirms expression of TASK1 and TASK3 in medulloblastoma cells. PMID: 20931182
    36. TASK-1 and 3 are determinant of aldosterone secretion and adrenocortical zonation. PMID: 20049674
    37. TASK-1 and TASK-3 differed insofar as a large portion of the C terminus was necessary for the full effects of halothane and TRH on TASK-3 but not on TASK-1 PMID: 11886861
    38. Renal Oat1 expression gradually increased throughout development, approaching adult levels at 30 days of age, where at days 40 and 45 Oat1 levels were greater in males than females PMID: 11907168
    39. C-PAF blocks TASK-1 or a closely related channel, the effect is PKC dependent, and the inhibition alters the electrical activity of myocytes in ways that would be arrhythmogenic in the intact heart. PMID: 12003807
    40. Association with p11 is essential for trafficking of TASK-1 to the plasma membrane. This association masks an ER retention signal identified as Lys-Arg-Arg that precedes the Ser-Ser-Val sequence. PMID: 12198146
    41. KCNK3 potassium channels are shown to bear two cytoplasmic trafficking motifs: an N-terminal dibasic site that binds beta-COP to hold channels in ER and a C-terminal "release" site that binds the ubiquitous intracellular regulator 14-3-3beta PMID: 12437930
    42. hOAT1-expressing cell line showed extensive para-aminohippuric acid transport. hOAT1 also demonstrated 1:1 coupling between organic anion and dicarboxylate. PMID: 12837685
    43. Host TASK-1 protein and HIV-1 Vpu undergo mutual functional destruction. PMID: 15099524
    44. TASK1 is expressed in pia mater, astrocytes, Purkinje and granule cells PMID: 15197476
    45. Human cytotrophoblast cells from term placenta are a site of expression for various K2P genes, two of which, namely, TASK1 and TREK1, are transcribed into protein. PMID: 15695101
    46. TASK-1 is hypoxia-sensitive and controls the resting membrane potential, thus implicating an important role for TASK-1 K+ channels in the regulation of pulmonary vascular tone. PMID: 16574908
    47. the role of TASK channels in sensing physiological stimuli. PMID: 17945357
    48. K(2)P channels as novel potassium conductance on T lymphocytes critically influencing T cell effector function and identify a possible molecular target for immunomodulation in T cell-mediated autoimmune disorders PMID: 18375952
    49. Thus, regulated expression of TASK channels might contribute to a molecular switch between death and survival of neurons in autoimmune CNS inflammation. PMID: 18824070
    50. ET-1 depolarized human primary pulmonary artery smooth muscle cells by phosphorylating TASK-1. This effect was abrogated by TASK-1 siRNA. This might represent a novel pathologic mechanism related to pulmonary arterial hypertension. PMID: 19188660

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  • 相關疾病：
    Pulmonary hypertension, primary, 4 (PPH4)
  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Two pore domain potassium channel (TC 1.A.1.8) family
  • 組織特異性：
    Widespread expression in adult. Strongest expression in pancreas and placenta. Lower expression in brain, lung, prostate, heart, kidney, uterus, small intestine and colon.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6278

    OMIM: 603220

    KEGG: hsa:3777

    STRING: 9606.ENSP00000306275

    UniGene: Hs.24040